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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

LC-ESI-IT-MS/MS and MALDI-TOF Approach: Identification of Natural Polymers from Rhizophora mangle Barks and Determination of Their Analgesic and Anti-inflammatory Properties

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Autor(es):
de Souza Mesquita, Leonardo Mendes [1] ; Bittencourt Rodrigues, Caroline Fabri [1] ; da Rocha, Claudia Quintino [1, 2] ; Bianchim, Mayara Silveira [1] ; Rodrigues, Clenilson Martins [3] ; de Oliveira, Vanda Maria [3] ; Gaeta, Henrique Hessel [1] ; Belchor, Mariana Novo [1] ; Toyama, Marcos Hikari [1] ; Vilegas, Wagner [1]
Número total de Autores: 10
Afiliação do(s) autor(es):
[1] UNESP Sao Paulo State Univ, Lab Bioprospect Nat Prod LBPN, Coastal Campus Sao Vicente, BR-11330900 Sao Paulo - Brazil
[2] UFMA Fed Univ Maranhao, LEAF, Ave Portugueses 1966 Bacanga, BR-65080805 Sao Luis, Maranhao - Brazil
[3] Brazilian Agr Res Corp, Embrapa Agroenergy, W3 Norte, PqEB, BR-70770901 Brasilia, DF - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: NATURAL PRODUCTS AND BIOPROSPECTING; v. 9, n. 1, p. 23-34, FEB 2019.
Citações Web of Science: 0
Resumo

We recognize the chemical composition of the acetonic extract of Rhizophora mangle barks (AERM) using mass spectrometry analysis {[}liquid chromatography (LC)-ESI-IT-MS/MS and matrix-assisted laser desorption/ionization-time of flight-MS (MALDI-TOF)]. Analgesic activity was evaluated by formalin and tail-flick experimental assays. Anti-inflammatory activity was performed by paw edema test induced by carrageenan and 48/80 compounds. The first series of experiments involved {[}LC]-FIA-IT-MS/MS with 11 separated catechins derivatives until degree of polymerization 3 (DP3). The spectra obtained by MALDI-TOF analysis of the AERM presented two homologous series: one based on polymers of m/z 288 Da increments (up to DP12) and another series based on polymers of m/z {[}288 + 162] Da increments (up to DP11). In addition to these series of flavan-3-ol, each DP had a subset of masses with a variation of - 16 Da (homologous series of afzelechins-m/z 873-3465 Da) and + 16 Da (homologous series of gallocatechins-m/z 905-3497 Da). A similar pattern with homologous series of gallocatechins and afzelechins could also be observed for a fifth and a sixth monohexoside series: glucogallocatechins (m/z 779-3371) and glucoafzelechins (m/z 747-3339). The intraperitoneal administration of different doses of AERM (50, 150 and 300 mu g mL(-1)) have a morphine-like effect and intense anti-inflammatory activity. (AU)

Processo FAPESP: 14/23951-3 - Fitoterápicos padronizados para o tratamento de doenças crônicas: Rhizophora mangle
Beneficiário:Leonardo Mendes de Souza Mesquita
Linha de fomento: Bolsas no Brasil - Mestrado
Processo FAPESP: 09/52237-9 - Fitoterápicos padronizados como alvo para o tratamento de doenças crônicas
Beneficiário:Wagner Vilegas
Linha de fomento: Auxílio à Pesquisa - Programa BIOTA - Temático