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(Referência obtida automaticamente do SciELO, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Lack of association among TNF-α gene expression, -308 polymorphism (G > A) and virulence markers of Helicobacter pylori

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Luanna Munhoz Zabaglia ; Mariane Avante Ferraz ; Weendelly Nayara Pereira ; Wilson Aparecido Orcini ; Roger Willian de Labio ; Agostinho Caleman Neto ; Fernanda Wisnieski ; Juliana Garcia de Oliveira ; Marilia de Arruda Cardoso Smith ; Spencer Luiz Marques Payão ; Lucas Trevizani Rasmussen
Número total de Autores: 11
Tipo de documento: Artigo Científico
Fonte: Journal of Venomous Animals and Toxins including Tropical Diseases; v. 21, p. -, 2015.
Resumo

Abstract Background Tumor necrosis factor plays a critical role in the pathogenesis of gastric diseases such as gastric cancer, and an abnormal inflammatory response has frequently been observed in dyspeptic patients. Helicobacter pylori infection can induce a gastric mucosal inflammatory response that may be influenced by -308 (G > A) polymorphisms and gene expression of theTNF-α gene. Methods One hundred and thirty-four gastric biopsy samples were collected from patients of both genders (61♂ and 73♀, mean age 40.3 ± 24.2 years) with gastric symptoms. The -308 (G > A) polymorphism of TNF-α was characterized using polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP). The expression level was measured using real-time PCR, and relative quantification (RQ) was calculated using the comparative CT method (2-ΔΔCT). Results The analysis revealed an increase in TNF-α gene expression in patients with gastritis; on the other hand, no statistical differences were observed in patients with gastric cancer. In addition, no association was found among -308 polymorphism genotypes, virulence markers, or TNF-α gene expression. Conclusions Helicobacter pylori induces a large increase in TNF-α expression in patients with gastritis, regardless of tissue inflammation, but after the tissue becomes neoplastic, the presence of bacteria did not influence expression. These results suggest that the TNF-α pathway may play an important role in the progression from gastritis to gastric cancer (AU)

Processo FAPESP: 13/21224-4 - Genotipagem e análise da expressão do gene tnf-alpha em amostras de pacientes com sintomas pépticos e câncer gástrico: uma possível relação com o helicobacter pylori
Beneficiário:Luanna Munhoz Zabaglia
Linha de fomento: Bolsas no Brasil - Iniciação Científica
Processo FAPESP: 12/18333-3 - Caracterização do marcador de patogenicidade dupA, do Helicobacter pylori, genotipagem e análise da expressão dos genes do fator de necrose tumoral e E-caderina em amostras de crianças e adultos com sintomas pépticos
Beneficiário:Lucas Trevizani Rasmussen
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 13/21285-3 - Caracterização do Marcador de Patogenicidade dupA, do Helicobacter pylori e Análise da Expressão do Gene da E-caderina em Amostras de Biopsia Gástrica de Pacientes Dispépticos e com Adenocarcinoma Gástrico
Beneficiário:Mariane Avante Ferraz
Linha de fomento: Bolsas no Brasil - Iniciação Científica