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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Phenotypic, molecular and genomic characterization of Actinobaculum suis isolated from swine in Brazil

Texto completo
Autor(es):
Amigo, Cristina Roman [1] ; Moreno, Luisa Zanolli [1, 2] ; Porfida Ferreira, Thais Sebastiana [1] ; de Moura Gomes, Vasco Tulio [1] ; Poor, Andre Pegoraro [1] ; de Oliveira, Carolina Helena [1] ; Parra, Beatriz Martins [1] ; Santos Silva, Ana Paula [1] ; Moreno, Andrea Micke [1]
Número total de Autores: 9
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Prevent Vet Med & Anim Hlth, Sao Paulo - Brazil
[2] Ctr Univ Max Planck UniMax, Indaiatuba, SP - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: Anaerobe; v. 56, p. 27-33, APR 2019.
Citações Web of Science: 0
Resumo

Urinary tract infections (UTI) are considered one of the most important diseases of sows due to its close relationship with reproductive problems such as reduced litter size, increase in the rate of return to estrous, vulvar discharge, abortion, mastitis and anestrus. Actinobaculum suis is one of the main agents involved in porcine urinary tract infection and is responsible for the most severe and fatal cases in sows. In the present report, 23 A. suis strains isolated from a sow and boars in Brazil were identified by PCR and further characterized by broth microdilution, molecular typing by pulsed-field gel electrophoresis (PFGE), single-enzyme amplified fragment length polymorphism (SE-AFLP), and whole-genome sequencing. All strains were sensitive to ceftiofur, linezolid, nitrofurantoin, quinupristin-dalfopristin and vancomycin. Ciprofioxacin, daptomycin, lincomycin, erythromycin and tylosin resistance was observed in 100% of tested strains. Tetracycline and tigecycline also presented high resistance rates (87% and 30.4%, respectively). PFGE with eight different restriction enzymes and three programs did not enable strain characterization; however, all strains were typed by SE-AFLP that clustered strains according to their origin, thus proving an effective tool for A. suis genotyping. Whole-genome sequencing and comparative analysis enabled species differentiation from closely related genus. This is the first report of genomic characterization of A. suis. (C) 2019 Elsevier Ltd. All rights reserved. (AU)

Processo FAPESP: 17/09515-4 - Identificação e caracterização fenotípica de patógenos bacterianos causadores de metrite em fêmeas suínas.
Beneficiário:André Pegoraro Poor
Linha de fomento: Bolsas no Brasil - Mestrado
Processo FAPESP: 16/25745-7 - Avaliação da virulência e caracterização fenotípica e genômica de estirpes de Corynebacterium spp. isoladas de infecção urinária de suínos
Beneficiário:Luisa Zanolli Moreno
Linha de fomento: Bolsas no Brasil - Pós-Doutorado