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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

In vitro modulation of human gut microbiota composition and metabolites by Bifidobacterium longum BB-46 and a citric pectin

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Autor(es):
Bianchi, Fernanda [1, 2] ; Larsen, Nadja [2] ; Tieghi, Thatiana de Mello [1] ; Adorno, Maria Angela T. [3] ; Saad, I, Susana M. ; Jespersen, Lene [2] ; Sivieri, Katia [1]
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] State Univ Sao Paulo UNESP, Sch Pharmaceut Sci, Dept Food & Nutr, BR-14801902 Araraquara, SP - Brazil
[2] Univ Copenhagen, Fac Sci, Dept Food Sci, DK-1958 Frederiksberg C - Denmark
[3] Univ Sao Paulo, Sch Engn Sao Carlos, Dept Hydraul & Sanitat, BR-13563120 Sao Carlos, SP - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: Food Research International; v. 120, p. 595-602, JUN 2019.
Citações Web of Science: 0
Resumo

The gut microbiota composition and its metabolites have high impact on human health. Exploitation of prebiotics and probiotics for modulation of gut microbiota can lead to promising outcomes. This study aimed to evaluate the effects of the probiotic strain Bifidobacterium longum BB-46 alone and in combination with a citric pectin from lemon on the gut microbiota from healthy adults using the Simulator of Human Intestinal Microbial Ecosystem (SHIME center dot). Changes in microbiota composition and in metabolic activity were assessed by the 16S rRNA gene sequencing and by analyses of short-chain fatty acids (SCFAs) and ammonium ions (NH4+). An increase in the relative abundances of Firmicutes (especially the members of Lachnospiraceae and Lactobacillaceae families) and Bacteroidetes was observed during treatment with B. longum BB-46 alone in all compartments of the colon. Treatment with B. longum BB-46 and pectin stimulated an increase in the proportions of genera Faecalibacteriwn, Eubacterium and Lactobacillus, as well as in the Ruminococcaceae family in the transverse and descending colons. Concurrently, the butyrate levels increased in these two compartments. Additionally, the combination of B. longum BB-46 and pectin reduced the abundance of proteolytic bacteria Bacteroides, Clostridium, Peptoniphilus, and Streptococcus, along with decreased NH4+ production. No significant changes could be observed on NH4+ production by treatment with B. longum BB-46, nor did it increase the amount of SCFAs. In this study, we observed that although each treatment was able to modulate the microbiota, the combination of B. longum BB-46 and pectin was more efficient in decreasing the intestinal NH(4)(+)d levels and in increasing butyric acid-producing bacteria. These findings indicate that B. longwn BB-46, especially when combined with the specific citric pectin, might have beneficial impact on human health. (AU)

Processo FAPESP: 15/13965-0 - Seleção e avaliação da combinação entre resíduos de frutas tropicais e cepas probióticas na modulação da microbiota intestinal de indivíduos obesos em relação à de eutróficos utilizando simulador do ecossistema microbiano humano
Beneficiário:Katia Sivieri
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 15/08228-6 - Seleção e avaliação da combinação entre resíduos de frutas tropicais e cepas probióticas na modulação da microbiota intestinal de indivíduos obesos em relação à de eutróficos utilizando simulador do ecossistema microbiano humano.
Beneficiário:Fernanda Bianchi
Linha de fomento: Bolsas no Brasil - Doutorado
Processo FAPESP: 13/50506-8 - Bioactive components from by-products of food processing used in a symbiotic approach for improving human health and well-being (BioSyn)
Beneficiário:Susana Marta Isay Saad
Linha de fomento: Auxílio à Pesquisa - Temático
Processo FAPESP: 16/20336-1 - Efeito da atividade simbiótica sob a integridade do epitélio intestinal em microbiota obesa e eutrófica
Beneficiário:Fernanda Bianchi
Linha de fomento: Bolsas no Exterior - Estágio de Pesquisa - Doutorado