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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Vascular endothelial growth factor C treatment for mouse hind limb lymphatic revascularization

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Autor(es):
Ferrao, Juliana S. P. [1] ; Bonfim Neto, Antenor P. [1] ; da Fonseca, Vanessa U. [1] ; Sousa, Liza M. M. de C. [2] ; Papa, Paula de C. [1]
Número total de Autores: 5
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Dept Surg, Sch Vet Med & Anim Sci, Sao Paulo - Brazil
[2] Univ Paulista, Hlth Sci Inst, Sao Paulo - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: VETERINARY MEDICINE AND SCIENCE; v. 5, n. 2, p. 249-259, MAY 2019.
Citações Web of Science: 0
Resumo

Spontaneous lymphatic revascularization is a challenge and the establishment of new therapeutic strategies may improve life quality for patients suffering from lymphatic disorders. This study was designed to verify if VEGFC treatment improves lymphatic vascularization in a time-dependent manner in mouse hindlimb (HL) after resection of the inguinal lymph node. Lymphatic vascular density (Vv) and length (Lv) were evaluated by stereology after immunohistochemistry. The control Group (CG) was not manipulated but received saline instead of VEGFC treatment. The surgery Group (SG) had the left inguinal lymph node resected but did not received VEGFC treatment. VEGFC Treated Group (TG) had the node resected and received VEGFC treatment. VEGFC and VEGFR3 local expression were assessed by qPCR. There was an effect of time over Vv and Lv in the SG and significant difference between CG and SG in the regions studied (proximal, medium and distal regions) of the left HL (LHL). The Lv showed significant difference between CG and SG only in the medium region. The Vv and the Lv for TG were higher than the other groups. VEGFC and VEGFR3 gene expression presented time effect in all regions of the LHL for SG and TG. Both VEGFC and VEGFR3 gene expression presented significant difference between CG and SG, between SG and TG and between CG and TG. This study showed significant decrease in lymphatic vascularization in the left hindlimb of mice after surgical removal of the inguinal lymph node and adjacent lymphatic vessels. Exogenous VEGFC could recover lymphatic vascularization through stimulating neolymphangiogenesis. (AU)

Processo FAPESP: 11/04538-0 - VEGF-C no tratamento de linfedema
Beneficiário:Juliana Shimara Pires Ferrao
Modalidade de apoio: Bolsas no Brasil - Mestrado