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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

A Thermostable Aspergillus fumigatus GH7 Endoglucanase Over-Expressed in Pichia pastoris Stimulates Lignocellulosic Biomass Hydrolysis

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Autor(es):
Bernardi, Aline Vianna [1] ; Yonamine, Deborah Kimie [1] ; Uyemura, Sergio Akira [2] ; Dinamarco, Taisa Magnani [1]
Número total de Autores: 4
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Chem Dept, Fac Philosophy Sci & Literature Ribeirao Preto, BR-14040901 Ribeirao Preto - Brazil
[2] Univ Sao Paulo, Dept Clin Toxicol & Bromatol Anal, Fac Pharmaceut Sci, BR-14040903 Ribeirao Preto - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES; v. 20, n. 9 MAY 1 2019.
Citações Web of Science: 0
Resumo

In the context of avoiding the use of non-renewable energy sources, employing lignocellulosic biomass for ethanol production remains a challenge. Cellulases play an important role in this scenario: they are some of the most important industrial enzymes that can hydrolyze lignocellulose. This study aims to improve on the characterization of a thermostable Aspergillus fumigatus endo-1,4--glucanase GH7 (Af-EGL7). To this end, Af-EGL7 was successfully expressed in Pichia pastoris X-33. The kinetic parameters K-m and V-max were estimated and suggested a robust enzyme. The recombinant protein was highly stable within an extreme pH range (3.0-8.0) and was highly thermostable at 55 degrees C for 72 h. Low Cu2+ concentrations (0.1-1.0 mM) stimulated Af-EGL7 activity up to 117%. Af-EGL7 was tolerant to inhibition by products, such as glucose and cellobiose. Glucose at 50 mM did not inhibit Af-EGL7 activity, whereas 50 mM cellobiose inhibited Af-EGL7 activity by just 35%. Additionally, the Celluclast((R)) 1.5L cocktail supplemented with Af-EGL7 provided improved hydrolysis of sugarcane bagasse in natura, sugarcane exploded bagasse (SEB), corncob, rice straw, and bean straw. In conclusion, the novel characterization of Af-EGL7 conducted in this study highlights the extraordinary properties that make Af-EGL7 a promising candidate for industrial applications. (AU)

Processo FAPESP: 18/11231-7 - Caracterização funcional da endoglucanase GH7 de Aspergillus fumigatus
Beneficiário:Deborah Kimie Yonamine
Linha de fomento: Bolsas no Brasil - Iniciação Científica
Processo FAPESP: 14/10466-0 - Análise transcricional do fungo Aspergillus fumigatus quando crescido em bagaço de cana-de-açúcar
Beneficiário:Taisa Magnani Dinamarco
Linha de fomento: Auxílio à Pesquisa - Programa BIOEN - Regular