Polyalthic Acid in Polymeric Nanoparticles Causes Selective Growth Inhibition and Genotoxicity in MCF-7 Cells

Autor(es)

Chibas, Leniher Castan ^[1] ; Cintra, Priscila Pavini ^[1] ; Moreira, Monique Rodrigues ^[1] ; Goulart, Mirian Oliveira ^[1] ; Ambrosio, Sergio Ricardo ^[1] ; Sola Veneziani, Rodrigo Cassio ^[1] ; Bastos, Jairo Kenupp ^[2] ; dos Santos, Raquel Alves ^[1]

Afiliação do(s) autor(es)

^[1] Univ Franca, Nucleo Pesquisas Ciencias Exatas & Tecnol, Ave Dr Armando Salles de Oliveira 201, BR-14404600 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: NATURAL PRODUCT COMMUNICATIONS; v. 14, n. 4 APR 2019.
• Citações Web of Science: 0

Resumo

Polyalthic acid (PA) is a diterpene present in several trees of the Copaifera genus, with reported antitumor activity but poor water solubility. The aim of this work was the incorporation of PA in polymeric nanoparticles and the evaluation of the antiproliferative activity of this formulation in tumor (MCF-7) and normal (MCF-10A) breast cell lines. The nanoparticles were obtained by nanoprecipitation, using poly lactic-co-glycolic acid (PLGA) as the main material. Scanning electron microscopy showed nanoparticles with semispherical morphology, and dynamic light scattering measures revealed negative surface charge and average size of 98.64 +/- 28 nm. The encapsulation efficiency was 98% and the drug loading was 15.6% +/- 0.02%. Treatments with PA nanoparticles reduced cell proliferation more efficiently than free PA and the effect was selective on MCF-7 cells. Comet assay revealed a selective DNA damage induction by the nanoformulation on the tumor cells, which probably caused the antiproliferative effect. Our results show that PA incorporated in PLGA nanoparticles has potential as a selective cytostatic and genotoxic agent against MCF-7 cells. (AU)

• Processo FAPESP: 11/13630-7 - Validação química e farmacológica de extratos e princípios ativos de espécies de Copaifera
• Beneficiário: Jairo Kenupp Bastos
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 14/12465-0 - Avaliação das vias de resposta a danos no DNA moduladas por estilbenos estruturalmente modificados
• Beneficiário: Raquel Alves dos Santos
• Modalidade de apoio: Auxílio à Pesquisa - Regular