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Genetic Diversity of `Candidatus Liberibacter asiaticus' Revealed by Short Tandem Repeats and Prophage Typing Indicates Population Homogeneity in Brazil

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Autor(es):
da Silva, Priscila Alves [1] ; Fassini, Camila Giacomo [1] ; Sampaio, Lais Simes [1] ; Dequigiovanni, Gabriel [2] ; Zucchi, Maria Imaculada [2] ; Wulff, Nelson Arno [3, 1]
Número total de Autores: 6
Afiliação do(s) autor(es):
[1] Dept Pesquisa & Desenvolvimento Fundecitrus, BR-14807040 Araraquara, SP - Brazil
[2] Agencia Paulista Tecnol Agronegocios, Rodovia SP 127, Km 30, BR-13400970 Piracicaba, SP - Brazil
[3] IQ UNESP, PPG Biotecnol, BR-14800060 Araraquara, SP - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: PHYTOPATHOLOGY; v. 109, n. 6, p. 960-971, JUN 2019.
Citações Web of Science: 0
Resumo

`Candidatus Liberibacter asiaticus' is the most common huanglongbing-associated bacteria, being present in Asia, South, Central, and North America. Genomic approaches enabled sequencing of `Ca. L. asiaticus' genomes, allowing for a broader assessment of its genetic variability with the application of polymerase chain reaction (PCR)-based tools such as microsatellite or short tandem repeat (STR) analysis. Although these tools contributed to a detailed analysis of strains from Japan, China, and the United States, Brazilian strains were analyzed in either too few samples with several STRs or in several strains with only a single microsatellite and a single PCR marker. We used 573 `Ca. L. asiaticus' strains, mainly collected from Sdo Paulo State (SPS), in our genetic analyses, employing three STRs and several prophage PCR markers. STR revealed a homogeneous population regardless of sampling year or geographic regions of SPS. Thirty-eight haplotypes were recognized with a predominance of VNTR\_005 higher than 10 repeats, with VNTR\_002 and VNTR\_077 containing 11 and 8 repeats, respectively. This haplotype is indicated as class HE, which comprised 80.28% of strains. Classes HA and HB, predominant in Florida, were not found. A new genomic organization in the junction of prophages SC2 and SC1 is prevalent in Brazilian strains, indicating gene rearrangement and a widespread occurrence of a type 1 prophage as well as the presence of a type 2-like prophage. Our results indicate that `Ca. L. asiaticus' populations are homogeneous and harbor a new genomic organization in prophages type 1 and 2. (AU)

Processo FAPESP: 16/01993-1 - Análise da variabilidade de Candidatus Liberibacter asiaticus e produção de plantas cítricas com genes de profagos
Beneficiário:Priscila Alves da Silva
Linha de fomento: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 15/07011-3 - Estratégias biotecnológicas para o controle do HLB mediante transgenia
Beneficiário:Leandro Antonio Peña Garcia
Linha de fomento: Auxílio à Pesquisa - Temático