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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Anti-bacterial efficacy via drug-delivery system from layer-by-layer coating for percutaneous dental implant components

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Autor(es):
de Avila, Erica D. [1, 2] ; Castro, Antonio G. B. [1] ; Tagit, Oya [3, 4] ; Krom, Bastiaan P. [5, 6] ; Lowik, Dennis [7] ; van Well, Ad A. [8] ; Bannenberg, Lars J. [8] ; Vergani, Carlos Eduardo [2] ; van den Beucken, Jeroen J. J. P. [1]
Número total de Autores: 9
Afiliação do(s) autor(es):
[1] Radhoudumc, Regenerat Biomat, Philips van Leydenlaan 25, Nijmegen - Netherlands
[2] Sao Paulo State Univ Unesp, Dept Dent Mat & Prosthodont, Sch Dent Araraquara, Humaita 1680, Araraquara, SP - Brazil
[3] Radhoudumc, Dept Tumor Immunol, Geert Grootepl Zuid 28, Nijmegen - Netherlands
[4] Radhoud Inst Mol Life Sci, Geert Grootepl Zuid 28, Nijmegen - Netherlands
[5] Univ Amsterdam, Dept Prevent Dent, Acad Ctr Dent Amsterdam ACTA, Gustav Mahlerlaan 3004, Amsterdam - Netherlands
[6] Vrije Univ Amsterdam, Gustav Mahlerlaan 3004, Amsterdam - Netherlands
[7] Radhoud Univ, Dept Organ Chem, Heyendaalseweg 135, Nijmegen - Netherlands
[8] Delft Univ Technol, Fac Appl Sci, Lorentzweg 1, Delft - Netherlands
Número total de Afiliações: 8
Tipo de documento: Artigo Científico
Fonte: Applied Surface Science; v. 488, p. 194-204, SEP 15 2019.
Citações Web of Science: 1
Resumo

Percutaneous medical devices are prone to bacterial contamination that causes dramatic clinical conditions. At the percutaneous level of dental implant systems, microbial pathogens induce biofilm formation that may result in bone resorption and dental implant loss. In view of peri-implantitis caused by bacterial inflammation at the percutaneous abutment region, we here establish a novel drug release system based on layer-by-layer (LbL)-deposited poly(acrylic acid) (PAA) and poly-L-lysine (PLL) coatings on titanium (Ti). Detailed multilayer coating characterization was performed by different microscopy and spectroscopy techniques to probe physical and chemical properties. Our data revealed a significant difference in roughness average between ten double layers coated (141 nm +/- 30) and uncoated Ti discs (115 nm +/- 40). Although roughness of the coatings increased significantly after immersion in water for 24 h at 37 degrees C, this physical property remained below 200 nm. Coating stability was confirmed under neutral and acidic pH, mimicking healthy and diseased/ inflammatory environments, respectively. LbL coatings supported in vitro human keratinocytes growth, demonstrating absence of cytotoxic effects. Tetracycline (TC) showed an initial burst release under neutral and acidic conditions, which further demonstrated robust antibacterial efficacy against Porphyromonas gingivalis. However, a convenient pH-dependent 2-folds increase in TC release was observed for coatings incubated at pH = 4.5. Sustained TC release was observed from coatings up till 15 days of incubation in both pH conditions. These results demonstrate the potential application of this simple surface modification to leverage anti-bacterial efficacy at the percutaneous abutment region. (AU)

Processo FAPESP: 15/03567-7 - Modificação das propriedades físico-químicas das superfícies de titânio para abutments de implantes com diferentes tipos de revestimentos e avaliação do efeito do tratamento no desenvolvimento de um complexo biofilme oral: um estudo preliminar in vitro
Beneficiário:Érica Dorigatti de Avila
Linha de fomento: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 16/19650-3 - Revestimento para abutments de implantes utilizando o método camada por camada: investigando o efeito da liberação de agentes antibacterianos na sinalização de citocinas e produção de mediadores inflamatórios induzidos por LPS de Porphyromonas gingivalis
Beneficiário:Érica Dorigatti de Avila
Linha de fomento: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado