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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

A Putative Role of Teneurin-2 and Its Related Proteins in Astrocytes

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Autor(es):
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Tessarin, Gestter W. L. [1, 2] ; Michalec, Ola M. [3] ; Torres-da-Silva, Kelly R. [1, 2] ; Da Silva, V, Andre ; Cruz-Rizzolo, Roelf J. [1] ; Goncalves, Alaide [1] ; Gasparini, Daniele C. [1] ; Horta-Junior, Jose A. C. [4] ; Ervolino, Edilson [1] ; Bittencourt, Jackson C. [5] ; Lovejoy, David A. [3] ; Casatti, Claudio A. [1, 4]
Número total de Autores: 12
Afiliação do(s) autor(es):
[1] Sao Paulo State Univ UNESP, Dept Basic Sci, Sch Dent Aracatuba, Aracatuba - Brazil
[2] Sao Paulo State Univ UNESP, Dept Anat, Inst Biosci Botucatu, Botucatu, SP - Brazil
[3] Univ Toronto, Dept Cell & Syst Biol, Toronto, ON - Canada
[4] Da Silva, Andre, V, Sao Paulo State Univ UNESP, Dept Anat, Inst Biosci Botucatu, Botucatu, SP - Brazil
[5] Sao Paulo Univ USP, Dept Anat, Inst Biomed Sci, Sao Paulo - Brazil
Número total de Afiliações: 5
Tipo de documento: Artigo Científico
Fonte: FRONTIERS IN NEUROSCIENCE; v. 13, JUN 27 2019.
Citações Web of Science: 0
Resumo

Teneurins are type II transmembrane proteins comprised of four phylogenetically conserved homologs (Ten-1-4) that are highly expressed during neurogenesis. An additional bioactive peptide named teneurin C-terminal-associated peptide (TCAP-1-4) is present at the carboxyl terminal of teneurins. The possible correlation between the Ten/TCAP system and brain injuries has not been explored yet. Thus, this study examined the expression of these proteins in the cerebral cortex after mechanical brain injury. Adult rats were subjected to cerebral cortex injury by needle-insertion lesion and sacrificed at various time points. This was followed by analysis of the lesion area by immunohistochemistry and conventional RT-PCR techniques. Control animals (no brain injury) showed only discrete Ten-2-like immunoreactive pyramidal neurons in the cerebral cortex. In contrast, Ten-2 immunoreactivity was significantly up-regulated in the reactive astrocytes in all brain-injured groups (p < 0.0001) when compared to the control group. Interestingly, reactive astrocytes also showed intense immunoreactivity to LPHN-1, an endogenous receptor for the Ten-2 splice variant named Lasso. Semi-quantitative analysis of Ten-2 and TCAP-2 expression revealed significant increases of both at 48 h, 3 days and 5 days (p < 0.0001) after brain injury compared to the remaining groups. Immortalized cerebellar astrocytes were also evaluated for Ten/TCAP expression and intracellular calcium signaling by fluorescence microscopy after TCAP-1 treatment. Immortalized astrocytes expressed additional Ten/TCAP homologs and exhibited significant increases in intracellular calcium concentrations after TCAP-1 treatment. This study is the first to demonstrate that Ten-2/TCAP-2 and LPHN-1 are upregulated in reactive astrocytes after a mechanical brain injury. Immortalized cerebellar astrocytes expressed Ten/TCAP homologs and TCAP-1 treatment stimulated intracellular calcium signaling. These findings disclose a new functional role of the Ten/TCAP system in astrocytes during tissue repair of the CNS. (AU)

Processo FAPESP: 09/54141-9 - Aquisição de Microscópio Confocal a Laser Leica TCS SP5 e Microscópio Eletrônico de Transmissao TECNAI Spirit
Beneficiário:Silvia Rodrigues Machado
Linha de fomento: Auxílio à Pesquisa - Programa Equipamentos Multiusuários
Processo FAPESP: 13/26779-4 - Efeito do zoledronato sobre o processo de reparo alveolar: estudo envolvendo os principais fatores de risco para o desenvolvimento de osteonecrose dos maxilares e avaliação da terapia fotodinâmica antimicrobiana (aPDT) como proposta preventiva
Beneficiário:Edilson Ervolino
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 10/52068-0 - A participação do hormônio concentrador de melanina no controle da lactação
Beneficiário:Jackson Cioni Bittencourt
Linha de fomento: Auxílio à Pesquisa - Temático
Processo FAPESP: 12/08833-9 - Expressão das teneurinas (TENS) e dos peptídeos associados ao terminal carboxila das teneurinas (TCAPs) no sistema nervoso central de primatas não-humanos (Cebus apella)
Beneficiário:Kelly Regina Torres da Silva
Linha de fomento: Bolsas no Brasil - Doutorado
Processo FAPESP: 08/02771-6 - Estudo hodológico, neuroquímico e comportamental das aferências ao primeiro núcleo do circuito neural do reflexo auditivo de sobressalto: núcleo da raiz coclear
Beneficiário:José de Anchieta de Castro e Horta Júnior
Linha de fomento: Auxílio à Pesquisa - Apoio a Jovens Pesquisadores