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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Standardization of a resazurin-based assay for the evaluation of metabolic activity in oral squamous carcinoma and glioblastoma cells

Texto completo
Autor(es):
Rezende, Nayara [1] ; Jayme, Cristiano Ceron [2, 1] ; Brassesco, Maria Sol [3] ; Tedesco, Antonio Claudio [2, 1] ; de Oliveira, Harley Francisco [4]
Número total de Autores: 5
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Fac Philosophy Sci & Letters Ribeirao Preto, Photobiol & Photomed Res Croup, Dept Chem, Ctr Nanotechnol & Tissue Engn, BR-14040901 Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, BR-14040901 Ribeirao Preto, SP - Brazil
[3] Univ Sao Paulo, Fac Philosophy Sci & Letters Ribeirao Preto, Lab Cell Biol & Oncogenet, Dept Biol, BR-14040901 Ribeirao Preto, SP - Brazil
[4] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Med Clin Radiotherapy Serv, BR-14048900 Ribeirao Preto, SP - Brazil
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: Photodiagnosis and Photodynamic Therapy; v. 26, p. 371-374, JUN 2019.
Citações Web of Science: 0
Resumo

The widely accepted resazurin-based assay can be used, prior to in vivo studies, as an inexpensive method to determine cytotoxicity. The aim of this study was to evaluate and standardize the assay conditions for oral squamous cancer cell (OSCC) and glioblastoma (U87-MG) lines by UV-vis spectroscopy. The cells were treated with 25 mu gmL(-1) of resazurin sodium salt and then incubated for 4 h, 6 h, and 6.5 h. All absorbance measurements were carried out at 21 +/- 1 degrees C on a spectrophotometer with a microplate reader. After 4-hs of incubation, resazurin was completely reduced by OSCC cells, as demonstrated by the suppression of the absorbance at 380 nm. However, the U87-MG cells needed 6.5 h of incubation to demonstrate the same behavior. The Statistical analysis did not indicate significant differences between the OSCC and U87-MG cell lines' viability after 4 and 6.5 h respectively. We concluded that spectroscopic analysis is an efficient method for the standardization of the resazurin assay. In addition, without the implementation of suitable protocols, there could be an increase in the chance of errors or false positives or negatives that would reduce the usefulness of the data. (AU)

Processo FAPESP: 18/10237-1 - Desenvolvimento e caracterização de scaffolds à base de PCL - PGE e DNA aplicados à engenharia de tecidos para regeneração óssea
Beneficiário:Cristiano Ceron Jayme
Linha de fomento: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 13/50181-1 - Utilização de nanocarreadores contendo fármacos fotossensibilizantes e outros ativos aplicados à terapia celular e tratamento de patologias do sistema nervoso central
Beneficiário:Antonio Claudio Tedesco
Linha de fomento: Auxílio à Pesquisa - Temático
Processo FAPESP: 14/09421-1 - Estudo dos quimioterápicos radiossensibilizadores cisplatina e temozolomida veiculados em sistemas de liberação de fármacos
Beneficiário:Harley Francisco de Oliveira
Linha de fomento: Auxílio à Pesquisa - Regular