Busca avançada
Ano de início
Entree
(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

In vitro osteogenesis process induced by hybrid nanohydroxyapatite/graphene nanoribbons composites

Texto completo
Autor(es):
Reis de Vasconcellos, Luana Marotta [1] ; do Prado, Renata Falchete [1] ; Sartori, Elisa Mattias [2, 3] ; Silveira Mendonca, Daniela Baccelli [2] ; Mendonca, Gustavo [2] ; Marciano, Fernanda Roberta [4] ; Lobo, Anderson Oliveira [4, 5]
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] Sao Paulo State Univ, Inst Sci & Technol, Dept Biosci & Oral Diag, Av Engn Francisco Jose Longo 777, BR-12245000 Sao Jose Dos Campos, SP - Brazil
[2] Univ Michigan, Sch Dent, Dept Biol & Mat Sci, Ann Arbor, MI 48109 - USA
[3] Sao Paulo State Univ, Sch Dent Aracatuba, Dept Surg & Integrated Clin, Aracatuba - Brazil
[4] Univ Brasil, Sci & Technol Inst, Rua Carolina Fonseca 584, BR-08230030 Sao Paulo, SP - Brazil
[5] Univ Fed Piaui, Postgrad Program Mat Sci & Engn, Interdisciplinary Lab Adv Mat, BR-64049550 Teresina, PI - Brazil
Número total de Afiliações: 5
Tipo de documento: Artigo Científico
Fonte: JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE; v. 30, n. 7 JUL 2019.
Citações Web of Science: 0
Resumo

Carbon nanotubes combine high bend and mechanical strength, which is advantageous for many structural and biomedical purposes. Recently, some biomaterials, based on carbon nanostructures and nanohydroxyapatite (nHAp), have been investigated as bone substitutes in order to improve regeneration. The aim of this study was to access the expression of some RNA transcripts (involved in the process of osteoblast differentiation) by mesenchymal stem cells cultured over different nanocomposite surfaces. A multi-walled carbon nanotube (MWCNT) was firstly grown using chemical vapor deposition and then exfoliated using chemical and oxygen plasma treatments to obtain graphene nanoribbons (GNR). The hybrid composites nHAp/GNR were prepared using the wet method assisted by ultrasound irradiation with different amounts of GNR (1.0, 2.0 and 3.0wt %). Five groups were tested in cell cultures. Group 1: synthesized nHAp; Group 2: synthesized GNR; Group 3: nHAp and 1.0% of GNR; Group 4: nHAp and 2.0% of GNR and group 5: nHAp and 3.0% of GNR. Real time reverse transcription polymerase chain reactions were performed, and all data was submitted to Kruskal Wallis and Dunn tests, at a significance level of 5%. As a result, three nanocomposites with different proportions of GNR were successfully produced. After cell culture, the expression of osteogenic genes demonstrated no significant differences among the groups and periods. However, bone morphogenetic protein II (BMP II), integrin binding sialoprotein (IBSP), and Osterix highest expressions were observed in the group containing 3.0% of GNR. In conclusion, our hybrid composites may be useful in bone interventions requiring mesenchymal stem cell differentiation into osteoblasts for healing. {[}GRAPHICS] . (AU)

Processo FAPESP: 11/20345-7 - Estudo de filmes de carbono-tipo diamante contendo nanopartículas incorporadas para aplicações biomédicas
Beneficiário:Fernanda Roberta Marciano
Linha de fomento: Auxílio à Pesquisa - Apoio a Jovens Pesquisadores
Processo FAPESP: 11/17877-7 - Desenvolvimento de novos scaffolds poliméricos por eletrofiação com incorporação de nanotubos alinhados e nanohidroxiapatita para regeneração óssea
Beneficiário:Anderson de Oliveira Lobo
Linha de fomento: Auxílio à Pesquisa - Apoio a Jovens Pesquisadores