Lytic Polysaccharide Monooxygenase from Aspergillus fumigatus can Improve Enzymatic Cocktail Activity During Sugarcane Bagasse Hydrolysis

Autor(es)

de Gouvea, Paula Fagundes ^[1] ; Gerolamo, Luis Eduardo ^[1] ; Bernardi, Aline Vianna ^[1] ; Soares Pereira, Lucas Matheus ^[1] ; Uyemura, Sergio Akira ^[2] ; Dinamarco, Taisa Magnani ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PROTEIN AND PEPTIDE LETTERS; v. 26, n. 5, p. 377-385, 2019.
• Citações Web of Science: 1

Resumo

Background: Lytic Polysaccharide Monooxygenases (LPMOs) are auxiliary accessory enzymes that act synergistically with cellulases and which are increasingly being used in second-generation bioethanol production from biomasses. Several LPMOs have been identified in various filamentous fungi, including Aspergillus fumigants. However, many LPMOs have not been characterized yet. Objective: To report the role of uncharacterized A. fumigatus AfAA9\_B LPMO. Methods: qRT-PCR analysis was employed to analyze the LPMO gene expression profile in different carbon sources. The gene encoding an AfAA9\_B (A fu4g07850) was cloned into the vector pET-28a(+), expressed in the E. coli strain Rosetta (TM) (DE3) pLysS, and purified by a Ni2+-nitrilotriacetic (Ni-NTA) agarose resin. To evaluate the specific LPMO activity, the purified protein peroxidase activity was assessed. The auxiliary LPMO activity was investigated by the synergistic activity in Celluclast 1.5L enzymatic cocktail. Results: LPMO was highly induced in complex biomass like sugarcane bagasse (SEB), Avicel (R) PH-101, and CM-cellulose. The LPMO gene encoded a protein comprising 250 amino acids, without a CBM domain. After protein purification, the AfAA9\_B molecular mass estimated by SDS-PAGE was 35 kDa. The purified protein specific peroxidase activity was 8.33+ 1.9 U g(-1). Upon addition to Celluclast 1.5L, Avicel (R) PH-101 and SEB hydrolysis increased by 18% and 22%, respectively. Conclusion: A. fumigatus LPMO is a promising candidate to enhance the currently available enzymatic cocktail and can therefore be used in second-generation ethanol production. (AU)

• Processo FAPESP: 18/10296-8 - Construção de uma cepa de S. cerevisiae, pelo sistema CRISPR/Cas9, capaz de expressar uma LPMO, visando o melhoramento da sacarificação de biomassas lignocelulósicas
• Beneficiário: Paula Fagundes de Gouvêa Bizzi
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 16/19095-0 - Identificação, caracterização e expressão funcional de enzimas auxiliares hidrolíticas do tipo LPMOs do fungo Aspergillus fumigatus
• Beneficiário: Taisa Magnani Dinamarco
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Regular