Analysis of 8q24.21 miRNA cluster expression and copy number variation in gastric cancer

Autor(es):Mostrar menos -

Anauate, Ana C. ^[1] ; Leal, Mariana F. ^[1] ; Wisnieski, Fernanda ^{[1, 2]} ; Santos, Leonardo C. ^[1] ; Gigek, Carolina O. ^{[1, 3]} ; Chen, Elizabeth S. ^[1] ; Calcagno, Danielle Q. ^[4] ; Assumpcao, Paulo P. ^[4] ; Demachki, Samia ^[4] ; Arasaki, Carlos H. ^[3] ; Artigiani, Ricardo ^[5] ; Rasmussen, Lucas T. ^[6] ; Payao, Spencer M. ^[6] ; Burbano, Rommel R. ^{[4, 7, 8]} ; Smith, Marilia A. C. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Dept Morfol & Genet, Disciplina Genet, BR-04023900 Sao Paulo - Brazil

^[2] Univ Fed Sao Paulo, Dept Med, Disciplina Gastroenterol, BR-04040002 Sao Paulo - Brazil

^[3] Univ Fed Sao Paulo, Dept Cirurgia, Disciplina Gastroenterol Cirug, BR-04024002 Sao Paulo - Brazil

^[4] Univ Fed Para, Hosp Univ Joao de Barros Barreto, Nucleo Pesquisas Oncol, BR-66073000 Belem, Para - Brazil

^[5] Univ Fed Sao Paulo, Dept Patol, BR-04023062 Sao Paulo - Brazil

^[6] Fac Med Marilia, Dept Genet & Biol Mol, BR-17519050 Marilia - Brazil

^[7] Univ Fed Para, Inst Ciencias Biol, Lab Citogenet Humana, BR-66075110 Belem, Para - Brazil

^[8] Hosp Ophir Loyola, Lab Biol Mol, BR-66063240 Belem, Para - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Future Medicinal Chemistry; v. 11, n. 9, p. 947-958, MAY 2019.
• Citações Web of Science: 3

Resumo

Aim: To analyze gene expression and copy number of five miRNAs (miR-1204, miR-1205, miR-1206, miR-1207 and miR-1208) localized in this chromosome region in gastric cancer (GC). Materials\& methods: 65 paired neoplastic and non-neoplastic specimens collected from GC patients and 20 non-neoplastic gastric tissues from cancer-free individuals were included in this study. The expression levels of the five miRNAs were accessed by real time qPCR and were correlated. Results: MiR-1207-3p, miR-1205, miR-1207-5p and miR-1208 were upregulated in approximately 50% of GC tumors in relation to those of adjacent non-neoplastic tissues. MiR-1205 expression was associated with gain of gene copies and was upregulated in adjacent non-neoplastic samples relative to external controls. Conclusion: The coexpression of the 8q24 miRNAs indicated the role of miR-1205 in the initiation of gastric cancer development. (AU)

• Processo FAPESP: 16/19953-6 - Genes modulados epigeneticamente poderão constituir alvos terapêuticos em pacientes com câncer gástrico?
• Beneficiário: Fernanda Wisnieski
• Linha de fomento: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 12/14768-5 - Afecções ortopédicas não traumáticas de ombro: aspectos genéticos e moleculares
• Beneficiário: Mariana Ferreira Leal
• Linha de fomento: Bolsas no Brasil - Apoio a Jovens Pesquisadores