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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Maternal IgG impairs the maturation of offspring intrathymic IL-17-producing gamma delta T cells: Implications for murine and human allergies

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Autor(es):
de-Oliveira, Marilia G. [1] ; Lira, Aline A. L. [1] ; Sgnotto, Fabio R. [2] ; Inoue, Amanda H. S. [1] ; Santos, Ludimila S. [3, 1] ; Nakamatsu, Bernardo Y. [3] ; Duarte, Alberto J. S. [4, 1] ; Leite-de-Moraes, Maria [5] ; Victor, Jefferson R. [3, 1]
Número total de Autores: 9
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Med Sch, Div Clin Dermatol, Lab Med Invest LIM 56, Av Dr Eneas de Carvalho Aguiar 500, 3rd Floor, BR-05403000 Sao Paulo - Brazil
[2] Univ Sao Paulo, Med Sch, Div Hematol, Sao Paulo - Brazil
[3] Laureate Int Univ, FMU, Div Environm Hlth, Sao Paulo - Brazil
[4] Univ Sao Paulo, Med Sch, Div Pathol, Sao Paulo - Brazil
[5] Univ Paris 05, Lab Immunoregulat & Immunopathol, INEM, CNRS UMR8253, INSERM, UMR1151, Paris - France
Número total de Afiliações: 5
Tipo de documento: Artigo Científico
Fonte: CLINICAL AND EXPERIMENTAL ALLERGY; v. 49, n. 7, p. 1000-1012, JUL 2019.
Citações Web of Science: 2
Resumo

Background The precise mechanism involved in the acquisition of the IL-17+ profile of gamma delta T cells, the ligands responsible for this change, and whether this default is acquired during intrathymic maturation need to be elucidated. Objective This study aimed to evaluate whether IL-17-producing gamma delta T cells are present in the airways of tolerant offspring from allergen-sensitized mothers and the possible implication of maternal IgG in the generation of these cells. Methods Female mice were immunized or not, and the allergic response, frequency of gamma delta T cell subsets and cytokine production of the offspring were analysed by flow cytometry. The effects of passive in vivo transfer of purified IgG were investigated in offspring. A translational approach was employed to analyse gamma delta T cells in the thymus and PBMCs from humans. Results Maternal immunization reduced the frequency of spontaneous IL-17-producing gamma delta T cells in the thymus, spleen and lung of offspring. This effect was mimicked by the in vivo treatment of females with purified IgG. IgG directly interacted with gamma delta T cell membranes. The modulatory effect of human IgG on human infant intrathymic and adult peripheral gamma delta T cells showed similarities to murine gamma delta T cells, which is rarely reported in the literature. Conclusions \& Clinical Relevance Together, our results reveal that IgG from potentially tolerant atopic mothers can influence offspring thymic IL-17-producing gamma delta T cell maturation. Furthermore, we suggest that IgG is an unprecedented modulatory factor of murine and human gamma delta T cells. These observations may support the future development of IgG-based immunoregulatory therapeutic strategies. (AU)

Processo FAPESP: 18/05181-7 - Avaliação translacional do efeito modulador e/ou regulador dos anticorpos IgG na maturação de linfócitos TCD4, TCD8, nTreg, Tgammadelta e B intra-tímicos de neonatos.
Beneficiário:Alberto José da Silva Duarte
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 17/18558-9 - Avaliação in vitro da influência do repertório IgG murino sobre a maturação de linfócitos B e Tgamma-delta com potencial regulador e/ou modulador
Beneficiário:Amanda Harumi Sabô Inoue
Linha de fomento: Bolsas no Brasil - Iniciação Científica