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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Cellular and sub-cellular Cu isotope fractionation in the human neuroblastoma SH-SY5Y cell line: proliferating versus neuron-like cells

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Autor(es):
Costas-Rodriguez, Marta [1] ; Colina-Vegas, Legna [1, 2] ; Solovyev, Nikolay [1] ; De Wever, Olivier [3] ; Vanhaecke, Frank [1]
Número total de Autores: 5
Afiliação do(s) autor(es):
[1] Univ Ghent, Dept Chem, Atom & Mass Spectrometry A&MS Res Unit, Campus Sterre, Krijgslaan 281-S12, B-9000 Ghent - Belgium
[2] Univ Fed Sao Carlos UFSCar, Dept Quim, Rodovia Washington Luis KM 235, CP 676, BR-13565905 Sao Carlos, SP - Brazil
[3] Univ Ghent, Dept Human Struct & Repair, Lab Expt Canc Res, Corneel Heymanslaan 10, B-9000 Ghent - Belgium
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: ANALYTICAL AND BIOANALYTICAL CHEMISTRY; v. 411, n. 19, SI, p. 4963-4971, JUL 2019.
Citações Web of Science: 2
Resumo

Cu isotope fractionation was investigated in the human neuroblastoma SH-SY5Y cell line, in a proliferating/tumor phase (undifferentiated cells), and in a differentiated state (neuron-like cells), induced using retinoic acid (RA). The SH-SY5Y cell line displays genetic aberrations due to its cancerous origin, but differentiation drives the cell line towards phenotypes suitable for the research of neurological diseases (e.g., Alzheimer's disease or Parkinson's disease). Cellular Cu distribution was first explored by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) imaging and, subsequently, Cu isotopic analysis was performed at cellular and sub-cellular levels via multi-collector inductively coupled plasma-mass spectrometry (MC-ICP-MS). The SH-SY5Y cells showed a re-distribution of intracellular Cu upon RA differentiation. Both undifferentiated and differentiated cells became systematically enriched in the light Cu-63 isotope with increasing intracellular Cu content. Differentiated neuron-like SH-SY5Y cells showed a heavier Cu isotopic composition (+0.3) than did the undifferentiated proliferating cells when exposed to Cu for 24h. However, after a longer exposure time (72h), no difference was observed between both cellular phenotypes. Mitochondrial fractions were enriched in the light Cu-63 isotope, compared to whole cells, for both undifferentiated and differentiated cells (no significant difference). The Cu isotopic composition of the remaining cell lysates was heavier than that of the whole cells and +0.2 heavier in the differentiated cells than in the undifferentiated cells. These results indicate that neuronal differentiation affects the Cu isotope fractionation accompanying Cu uptake in the cells, but this effect does not seem to be associated with the mitochondrial Cu pathway. Cu isotope fractionation can be an interesting tool for studying Cu metabolism at a (sub)-cellular level in functional neurons. (AU)

Processo FAPESP: 17/23254-9 - Determinação quantitativa e imagem celular de Cu, Ru e Pt em células individuais usando Ablação a laser acoplado ao ICP-MS
Beneficiário:Legna Andreina Colina Vegas
Linha de fomento: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado