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The complete mt genomes of Lutzia halifaxia, Lt. fuscanus and Culex pallidothorax (Diptera: Culicidae) and comparative analysis of 16 Culex and Lutzia mt genome sequences

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Autor(es):
Sun, Ling [1] ; Li, Ting-Jing [1] ; Fu, Wen-Bo [1] ; Yan, Zhen-Tian [1] ; Si, Feng-Ling [1] ; Zhang, Yu-Juan [1] ; Mao, Qi-Meng [1] ; Demari-Silva, Bruna [2] ; Chen, Bin [1]
Número total de Autores: 9
Afiliação do(s) autor(es):
[1] Chongqing Normal Univ, Coll Life Sci, Inst Entomol & Mol Biol, Chongqing Key Lab Vector Insects, Chongqing 401331 - Peoples R China
[2] Univ Sao Paulo, Fac Saude Publ, Dept Epidemiol, Ave Dr Arnaldo 715, Sao Paulo - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: PARASITES & VECTORS; v. 12, JUL 26 2019.
Citações Web of Science: 0
Resumo

BackgroundDespite the medical importance of the genus Culex, the mitochondrial genome (mt genome) characteristics of Culex spp. are not well understood. The phylogeny of the genus and particularly the generic status of the genus Lutzia and the subgenus Culiciomyia remain unclear.MethodsThe present study sequenced and analyzed the complete mt genomes of Lutzia halifaxia, Lutzia fuscanus and Cx. (Culiciomyia) pallidothorax and assessed the general characteristics and phylogenetics of all known 16 mt genome sequences for species in the genera Culex and Lutzia.ResultsThe complete mt genomes of Lt. halifaxia, Lt. fuscanus and Cx. pallidothorax are 15,744, 15,803 and 15,578 bp long, respectively, including 13 PCGs, 22 tRNAs, two tRNAs and a control region (CR). Length variations in the Culex and Lutzia mt genomes involved mainly the CR, and gene arrangements are the same as in other mosquitoes. We identified four types of repeat units in the CR sequences, and the poly-T stretch exists in all of these mt genomes. The repeat units of CR are conserved to different extent and provide information on their evolution. Phylogenetic analyses demonstrated that the Coronator and Sitiens groups are each monophyletic, whereas the monophyletic status of the Pipiens Group was not supported; Cx. pallidothorax is more closely related to the Sitiens and Pipiens groups; and both phylogenetics analysis and repeat unit features in CR show that Lutzia is a characteristic monophyletic entity, which should be an independent genus.ConclusionsTo our knowledge, this is the first comprehensive review of the mt genome sequences and taxonomic discussion based on the mt genomes of Culex spp. and Lutzia spp. The research provides general information on the mt genome of these two genera, and the phylogenetic and taxonomic status of Lutzia and Culiciomyia. (AU)

Processo FAPESP: 16/08551-4 - Genômica populacional de Anopheles (Kerteszia) cruzii (Diptera: Culicidae) associada a paisagens na Região do Vale do Ribeira, São Paulo, Brasil
Beneficiário:Bruna Demari e Silva
Linha de fomento: Bolsas no Brasil - Pós-Doutorado