| Texto completo | |
| Autor(es): |
Collaco, Rita de Cassia O.
[1, 2]
;
Hyslop, Stephen
[2]
;
Dorce, Valquiria A. C.
[3]
;
Antunes, Edson
[2]
;
Rowan, Edward G.
[1]
Número total de Autores: 5
|
| Afiliação do(s) autor(es): | [1] Univ Strathclyde, Strathclyde Inst Pharm & Biomed Sci, Robertson Trust Wing Bldg, Room 601, Glasgow G4 0RE, Lanark - Scotland
[2] State Univ Campinas UNICAMP, Fac Med Sci, Dept Pharmacol, Rua Tessalia Vieira de Camargo 126, BR-13083887 Campinas, SP - Brazil
[3] Butantan Inst, Div Sci Dev, Pharmacol Lab, Ave Vital Brazil 1500, BR-05503900 Sao Paulo, SP - Brazil
Número total de Afiliações: 3
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Neuropharmacology; v. 153, p. 41-52, JUL 15 2019. |
| Citações Web of Science: | 0 |
| Resumo | |
Scorpionism is frequently accompanied by a massive release of catecholamines and acetylcholine from peripheral nerves caused by neurotoxic peptides present in these venoms, which have high specificity and affinity for ion channels. Tityus bahiensis is the second most medically important scorpion species in Brazil but, despite this, its venom remains scarcely studied, especially with regard to its pharmacology on the peripheral (somatic and autonomic) nervous system. Here, we evaluated the activity of T. bahiensis venom on somatic neurotransmission using myographic (chick and mouse neuromuscular preparations), electrophysiological (MEPP, EPP, resting membrane potentials, perineural waveforms, compound action potentials) and calcium imaging (on DRG neurons and muscle fibres) techniques. Our results show that the major toxic effects of T. bahiensis venom on neuromuscular function are presynaptically driven by the increase in evoked and spontaneous neurotransmitter release. Low venom concentrations prolong the axonal action potential, leading to a longer depolarization of the nerve terminals that enhances neurotransmitter release and facilitates nerve-evoked muscle contraction. The venom also stimulates the spontaneous release of neurotransmitters, probably through partial neuronal depolarization that allows calcium influx. Higher venom concentrations block the generation of action potentials and resulting muscle twitches. These effects of the venom were reversed by low concentrations of TTX, indicating voltage-gated sodium channels as the primary target of the venom toxins. These results suggest that the major neuromuscular toxicity of T. bahiensis venom is probably mediated mainly by alpha- and beta-toxins interacting with presynaptic TTX-sensitive ion channels on both axons and nerve terminals. (AU) | |
| Processo FAPESP: | 16/11319-6 - Atividade do veneno de Tityus bahiensis e suas frações isoladas em preparações neuromusculares somáticas e autônoma. |
| Beneficiário: | Rita de Cássia de Oliveira Collaço |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |
| Processo FAPESP: | 16/23829-9 - Veneno do Tityus bahiensis e suas frações isoladas: um estudo eletrofisiológico e de imagem de cálcio em preparações nervo-músculo do sistema nervoso somático e autonômico. |
| Beneficiário: | Rita de Cássia de Oliveira Collaço |
| Modalidade de apoio: | Bolsas no Exterior - Estágio de Pesquisa - Doutorado |
| Processo FAPESP: | 17/15175-1 - Modulação da guanilato ciclase solúvel e dos níveis intracelulares de nucleotídeos cíclicos em órgãos do trato urinário inferior e próstata |
| Beneficiário: | Edson Antunes |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |