Quantification of Giardia and Cryptosporidium in surface water: a risk assessment and molecular characterization

Autor(es)

Bataiero, M. O. ^[1] ; Araujo, R. S. ^[2] ; Nardocci, A. C. ^{[3, 4]} ; Matte, M. H. ^[4] ; Sato, M. I. Z. ^{[2, 3]} ; Lauretto, M. S. ^{[3, 5]} ; Razzolini, M. T. P. ^{[3, 4]}

Afiliação do(s) autor(es)

^[1] Sanit Surveillance Ctr, Div Act Regarding Environm, Ave Dr Arnaldo 351 Anexo III, Sao Paulo, SP - Brazil

^[2] Environm Co State Sao Paulo, Dept Environm Anal, Av Prof Frederico Hermann Jr 345, Sao Paulo - Brazil

^[3] NARA Ctr Res Environm Risk Assessment, Av Dr Arnaldo 715, BR-01246904 Sao Paulo - Brazil

^[4] Univ Sao Paulo, Sch Publ Hlth, Av Dr Arnaldo 715, 1 Andar, BR-01246904 Sao Paulo - Brazil

^[5] Univ Sao Paulo, Sch Arts Sci & Humanities, R Arlindo Bettio 1000, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: WATER SCIENCE AND TECHNOLOGY-WATER SUPPLY; v. 19, n. 6, p. 1823-1830, SEP 2019.
• Citações Web of Science: 0

Resumo

This study aimed to estimate the annual probability of Giardia and Cryptosporidium infection for a population supplied by contaminated drinking water sources. Parasites were quantified by the USEPA Method 1623.1/2012. Annual risk was estimated using the quantitative microbial risk assessment (QMRA) approach. Genotyping was performed using specific primers based on the 18S rRNA gene for Cryptosporidium and gdh gene for Giardia. Giardia was detected in 83.3% of the samples (<0.1 to 8.6 cysts/L) and Cryptosporidium in 37.5% (<0.1 to 2 oocysts/L). In general, annual risk values for Giardia were 1 log higher when compared with those obtained for Cryptosporidium. Giardia intestinalis A and B were present as well as C. hominis and C. parvum. The lack of protection measures for the water supply catchment point put the population's health at risk. The results provide data to support decision-makers to take actions to improve environmental quality resulting in a positive impact on consumers' health. (AU)

• Processo FAPESP: 12/12750-1 - Quantificação de Giardia e Cryptosporidium em águas de abastecimento público e avaliação de risco de infecção via ingestão de água em município da Região Metropolitana de São Paulo (RMSP)
• Beneficiário: Maria Tereza Pepe Razzolini
• Modalidade de apoio: Auxílio à Pesquisa - Regular