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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

IgG from Non-atopic Individuals Induces In Vitro IFN-gamma and IL-10 Production by Human Intra-thymic gamma delta T Cells: A Comparison with Atopic IgG and IVIg

Texto completo
Autor(es):
Santos, Ludimila Souza [1] ; Sgnotto, Fabio da Ressureicao [2] ; Sabo Inoue, Amanda Harumi [1] ; Padreca, Archangelo Fernandes [3] ; Menghini, Ricardo Palamar [3] ; da Silva Duarte, Alberto Jose [1, 4] ; Victor, Jefferson Russo [3, 1]
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Sch Med, Div Clin Dermatol, Lab Med Invest LIM 56, Ave Dr Eneas de Carvalho Aguiar 500, 3rd Floor, BR-05403000 Sao Paulo - Brazil
[2] Univ Sao Paulo, Sch Med, Div Hematol, Sao Paulo - Brazil
[3] Laureate Int Univ, FMU, Div Environm Hlth, Sao Paulo - Brazil
[4] Univ Sao Paulo, Sch Med, Div Pathol, Sao Paulo - Brazil
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: ARCHIVUM IMMUNOLOGIAE ET THERAPIAE EXPERIMENTALIS; v. 67, n. 4, p. 263-270, AUG 2019.
Citações Web of Science: 2
Resumo

Matured in the thymus, gamma delta T cells can modulate the development of allergy in humans. The main gamma delta T cell subsets have been described as interleukin (IL)-17A or interferon (IFN)-gamma producers, but these cells can also produce other modulatory cytokines, such as IL-4 and IL-10. Here, we aimed to evaluate whether IgG can modulate the profile of cytokine production by gamma delta T cells during their maturation in the thymus and after its migration to peripheral tissues. Thymic tissues were obtained from 12 infants, and peripheral blood mononuclear cells (PBMCs) were obtained from adults (both groups without an atopic background). IgG was purified from atopic and non-atopic volunteers. Thymocytes and PBMCs were cultured with purified atopic or non-atopic IgG, and intracellular cytokine production and phenotype were assessed. Mock and IVIg conditions were used as controls. IgG from non-atopic individuals induced IFN-gamma and IL-10 production by thymic gamma delta T cells, and no effect was observed on peripheral gamma delta T cells. IL-17 production was inhibited by non-atopic IgG on thymic gamma delta T cells and augmented by atopic IgG on peripheral gamma delta T cells. Modulated thymic gamma delta T cells did not produce IFN-gamma and IL-10 simultaneously. We additionally evaluated the phenotype of intrathymic gamma delta T cells and observed that IgG from all groups could induce CD25 expression and could not influence the CD28 expression of these cells. This report describes evidence revealing that IgG may influence the production of IFN-gamma and IL-10 by intrathymic gamma delta T cells depending on the donor atopic state. This observation is unprecedented and needs to be considered in further studies in the IgG immunotherapy field. (AU)

Processo FAPESP: 18/05181-7 - Avaliação translacional do efeito modulador e/ou regulador dos anticorpos IgG na maturação de linfócitos TCD4, TCD8, nTreg, Tgammadelta e B intra-tímicos de neonatos.
Beneficiário:Alberto José da Silva Duarte
Linha de fomento: Auxílio à Pesquisa - Regular