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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Adaptive laboratory evolution of nanocellulose-producing bacterium

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Autor(es):
Vasconcellos, Vanessa M. [1, 2] ; Farinas, Cristiane S. [1, 2] ; Ximenes, Eduardo [3] ; Slininger, Patricia [4] ; Ladisch, Michael [3]
Número total de Autores: 5
Afiliação do(s) autor(es):
[1] Embrapa Instrumentat, Sao Carlos, SP - Brazil
[2] Univ Fed Sao Carlos, Grad Program Chem Engn, Sao Carlos, SP - Brazil
[3] Purdue Univ, Lab Renewable Resources Engn, Weldon Sch Biomed Engn Agr & Biol Engn, W Lafayette, IN 47907 - USA
[4] USDA, Bioenergy Res Unit Anchor, Natl Ctr Agr Utilizat Res, Peoria, IL - USA
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: Biotechnology and Bioengineering; v. 116, n. 8, p. 1923-1933, AUG 2019.
Citações Web of Science: 0
Resumo

Adaptive laboratory evolution through 12 rounds of culturing experiments of the nanocellulose-producing bacterium Komagataeibacter hansenii ATCC 23769 in a liquid fraction from hydrothermal pretreatment of corn stover resulted in a strain that resists inhibition by phenolics. The original strain generated nanocellulose from glucose in standard Hestrin and Schramm (HS) medium, but not from the glucose in pretreatment liquid. K. hansenii cultured in pretreatment liquid treated with activated charcoal to remove inhibitors also converted glucose to bacterial nanocellulose and used xylose as carbon source for growth. The properties of this cellulose were the same as nanocellulose generated from media specifically formulated for bacterial cellulose formation. However, attempts to directly utilize glucose proved unsuccessful due to the toxic character of the lignin-derived phenolics, and in particular, vanillan and ferulic acid. Adaptive laboratory evolution at increasing concentrations of pretreatment liquid from corn stover in HS medium resulted in a strain of K. hansenii that generated bacterial nanocellulose directly from pretreatment liquids of corn stover. The development of this adapted strain positions pretreatment liquid as a valuable resource since K. hansenii is able to convert and thereby concentrate a dilute form of glucose into an insoluble, readily recovered and value-added product-bacterial nanocellulose. (AU)

Processo FAPESP: 16/10636-8 - Da fábrica celular à biorrefinaria integrada Biodiesel-Bioetanol: uma abordagem sistêmica aplicada a problemas complexos em micro e macroescalas
Beneficiário:Roberto de Campos Giordano
Linha de fomento: Auxílio à Pesquisa - Programa BIOEN - Temático
Processo FAPESP: 14/19000-3 - Simplificação do processo de conversão de biomassa a etanol por meio da integração da produção de enzimas in-house
Beneficiário:Cristiane Sanchez Farinas
Linha de fomento: Auxílio à Pesquisa - Programa BIOEN - Regular