Genomic Organization of Repetitive DNA Elements and Extensive Karyotype Diversity of Silurid Catfishes (Teleostei: Siluriformes): A Comparative Cytogenetic Approach

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Ditcharoen, Sukhonthip ^[1] ; Carlos Bertollo, Luiz Antonio ^[2] ; Rab, Petr ^[3] ; Hnatkova, Eva ^[4] ; Molina, Wagner Franco ^[5] ; Liehr, Thomas ^[6] ; Tanomtong, Alongklod ^[1] ; Triantaphyllidis, Costas ^[7] ; Ozouf-Costaz, Catherine ^[8] ; Tongnunui, Sampan ^[9] ; Pengseng, Puan ^[10] ; Supiwong, Weerayuth ^[11] ; Aroutiounian, Rouben ^[12] ; Cioffi, Marcelo de Bello ^[2]

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^[1] Khon Kaen Univ, Fac Sci, Dept Biol, Tox Subst Livestock & Aquat Anim Res Grp, Khon Kaen 40002 - Thailand

^[2] Univ Fed Sao Carlos UFSCar, Dept Genet & Evolucao, Rodovia Washington Luiz Km 235, CP 676, BR-13565905 Sao Carlos, SP - Brazil

^[3] Czech Acad Sci, Inst Anim Physiol & Genet, Lab Fish Genet, Rumburska 89, Libechov 27721 - Czech Republic

^[4] Czech Univ Life Sci, Fac Agrobiol Food & Nat Resources, Dept Zool & Fisheries, Kamycka 129, Prague 16500 - Czech Republic

^[5] Univ Fed Rio Grande Norte UFRN, Ctr Biociencias, Dept Biol Celular & Genet, BR-59078970 Natal, RN - Brazil

^[6] Univ Hosp Jena, Inst Human Genet, D-07747 Jena - Germany

^[7] Aristotle Univ Thessaloniki, Sch Biol, Fac Sci, Dept Genet Dev & Mol Biol, Univ Campus, Thessaloniki 54124 - Greece

^[8] Sorbonne Univ, IBPS, Lab Evolut Paris Seine, Case 5, 7 Quai St Bernard, F-75952 Paris 05 - France

^[9] Mahidol Univ, Dept Conservat Biol, Kanchanaburi Campus, Sai Yok 71150, Kanchanaburi Pr - Thailand

^[10] Walailak Univ, Sch Agr Technol, Thasala 80160, Nakhon Si Thamm - Thailand

^[11] Khon Kaen Univ, Fac Appl Sci & Engn, Nong Khai Campus, Muang 43000, Nong Khai - Thailand

^[12] Yerevan State Univ, Dept Genet & Cytol, Yerevan 0025 - Armenia

• Tipo de documento: Artigo Científico
• Fonte: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES; v. 20, n. 14 JUL 2 2019.
• Citações Web of Science: 0

Resumo

The catfish family Siluridae contains 107 described species distributed in Asia, but with some distributed in Europe. In this study, karyotypes and other chromosomal characteristics of 15 species from eight genera were examined using conventional and molecular cytogenetic protocols. Our results showed the diploid number (2n) to be highly divergent among species, ranging from 2n = 40 to 92, with the modal frequency comprising 56 to 64 chromosomes. Accordingly, the ratio of uni- and bi-armed chromosomes is also highly variable, thus suggesting extensive chromosomal rearrangements. Only one chromosome pair bearing major rDNA sites occurs in most species, except for Wallago micropogon, Ompok siluroides, and Kryptoterus giminus with two; and Silurichthys phaiosoma with five such pairs. In contrast, chromosomes bearing 5S rDNA sites range from one to as high as nine pairs among the species. Comparative genomic hybridization (CGH) experiments evidenced large genomic divergence, even between congeneric species. As a whole, we conclude that karyotype features and chromosomal diversity of the silurid catfishes are unusually extensive, but parallel some other catfish lineages and primary freshwater fish groups, thus making silurids an important model for investigating the evolutionary dynamics of fish chromosomes. (AU)

• Processo FAPESP: 18/22033-1 - Uma abordagem intercontinental para a investigação da evolução cromossômica, diversidade genética e biogeografia na ordem Osteoglossiformes (Teleostei: osteoglossomorpha). Parte III
• Beneficiário: Marcelo de Bello Cioffi
• Linha de fomento: Auxílio à Pesquisa - Regular