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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Lysine metabolism and amino acid profile in maize grains from plants subjected to cadmium exposure

Texto completo
Autor(es):
Kato, Fabiana Hibary [1] ; Carvalho, Marcia Eugenia Amaral [1] ; Gaziola, Salete Aparecida [1] ; Piotto, Fernando Angelo [1] ; Azevedo, Ricardo Antunes [1]
Número total de Autores: 5
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, ESALQ, Dept Genet, CP 09, BR-13418900 Piracicaba, SP - Brazil
Número total de Afiliações: 1
Tipo de documento: Artigo Científico
Fonte: Scientia Agricola; v. 77, n. 1 2020.
Citações Web of Science: 2
Resumo

In general, the effects of Cadmium (Cd) on crop quality are evaluated solely by Cd accumulation in the edible plant parts; thus, the potential effects on several nutritional features are not often taken into account. This study evaluated Cd effects on the enzymatic activities of lysine (Lys) metabolism, storage protein and amino acid profile in maize. Stress parameters were also assessed. In vegetative organs, Cd was accumulated in the following decreasing order: roots > stems > leaves. Cadmium accumulation in grains decreased at grain maturity (from 0.25 to 0.07 mu g g(-1) DW). In leaves, neither hydrogen peroxide content nor superoxide dismutase, catalase, ascorbate peroxide and guaiacol peroxidase activities did not change in Cd-treated plants compared to control plants. Lipid peroxidation was not detected in immature grains and leaves of plants under Cd exposure, indicating that Cd accumulation mainly in the roots is a mechanism to avoid oxidative stress in aboveground parts of the plant. However, Lys metabolism in immature grains was modified, showing increases in the specific activities of aspartate kinase (AK) and dihydrodipicolinate synthase (DHDPS) after Cd exposure. AK sensitivity to Lys feedback inhibition increased, but decreased in DHDPS in Cd-treated plants, suggesting differential regulation for these enzymes. In mature grains, the Lys content did not change, while the proline content increased by 54 % in Cd-treated plants. This is the first report on Cd effects on amino acid profile, storage protein contents and enzymes from Lys metabolism in grains of a cereal plant species. (AU)

Processo FAPESP: 09/54676-0 - Estresse oxidativo induzido por metais: novas abordagens
Beneficiário:Ricardo Antunes de Azevedo
Linha de fomento: Auxílio à Pesquisa - Temático
Processo FAPESP: 12/23981-4 - Análise das possíveis alterações do metabolismo de lisina induzidas pelo cádmio em milho (Zea mays)
Beneficiário:Fabiana Hibary Kato Belini
Linha de fomento: Bolsas no Brasil - Doutorado
Processo FAPESP: 13/15217-5 - Abordagem integrada de parâmetros anatômicos, fisiológicos, bioquímicos e moleculares para o estudo de mecanismos de tolerância ao cádmio em acessos de tomateiro
Beneficiário:Marcia Eugenia Amaral de Carvalho
Linha de fomento: Bolsas no Brasil - Doutorado
Processo FAPESP: 15/26640-1 - Estudo dos mecanismos de tolerância ao cádmio em acessos de tomateiros: abordagens moleculares
Beneficiário:Marcia Eugenia Amaral de Carvalho
Linha de fomento: Bolsas no Exterior - Estágio de Pesquisa - Doutorado