Treatment of experimental periodontal disease with antimicrobial photodynamic therapy in nicotine-modified rats

Autor(es)

Garcia, Valdir Gouveia ^{[1, 2]} ; Fernandes, Leandro Araujo ^{[3, 1]} ; Macarini, Valmir Campos ^[1] ; de Almeida, Juliano Milanezi ^[1] ; Martins, Thiago Marchi ^[1] ; Bosco, Alvaro Francisco ^[1] ; Hitomi Nagata, Maria Jose ^[1] ; Cirelli, Joni Augusto ^[4] ; Theodoro, Leticia Helena ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ, Grp Res & Study Laser Dent GEPLO, Dept Surg, Div Periodont, Sao Paulo - Brazil

^[2] Educ Fdn Barretos UNIFEB, Dent Sch Barretos, Univ Ctr, Master Course, Barretos, SP - Brazil

^[3] Univ Fed Alfenas, Dept Clin & Surg, Alfenas, MG - Brazil

^[4] Sao Paulo State Univ, Dept Diag & Surg, Div Periodont, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF CLINICAL PERIODONTOLOGY; v. 38, n. 12, p. 1106-1114, DEC 2011.
• Citações Web of Science: 24

Resumo

Background: The aim of this study was to compare antimicrobial photodynamic therapy (aPDT) as an adjunctive treatment to scaling and root planing (SRP) for induced periodontitis in nicotine-modified rats. Material \& Methods: A total of 240 rats were evenly divided into two groups: C - saline solution treatment; N - nicotine treatment. Periodontal disease was induced in both groups at the first mandibular molar. After 7 days, the ligature was removed. All animals were submitted to SRP and were divided according to the following treatments: SRP - irrigation with saline solution; Toluidine Blue-O (TBO) - irrigation with phenothiazinium dye (100 mu g/ml); LLLT - laser irradiation (660 nm; 0.03 W; 4 J); and aPDT - TBO and laser irradiation. Ten animals in each group/treatment were euthanized at 7, 15 and 30 days. The histometric and immunohistochemical values were statistically analysed. Results: Intragroup analysis demonstrated that in both groups the aPDT treatment resulted in lower bone loss (BL) when compared to SRP in all experimental periods. Intergroup analysis demonstrated that aPDT treatment resulted in lower BL in Group N than in Group C treated with SRP in all experimental periods. Conclusion: Antimicrobial photodynamic therapy was an effective adjunctive treatment to SRP for induced periodontitis in nicotine-modified rats. (AU)

• Processo FAPESP: 08/04927-3 - Ação da terapia fotodinâmica no tratamento da doença periodontal experimentalmente induzida em ratos tratados ou não com nicotina: estudo histológico, histométrico e imunoistoquímico
• Beneficiário: Valdir Gouveia Garcia
• Modalidade de apoio: Auxílio à Pesquisa - Regular