Busca avançada
Ano de início
Entree
(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Advanced glycation endproducts produced by in vitro glycation of type I collagen modulate the functional and secretory behavior of dorsal root ganglion cells cultivated in two-dimensional system

Texto completo
Autor(es):
Bufalo, Michelle C. [1] ; Almeida, Maira E. [2] ; Franca, Isabella Araujo [2] ; Zambelli, Vanessa O. [1] ; Sant'anna, Morena Brazil Martins [1] ; Kimura, Louise F. [1] ; Giardini, Aline Carolina [1] ; Cury, Yara [1] ; Sampaio, Sandra Coccuzzo [3, 2]
Número total de Autores: 9
Afiliação do(s) autor(es):
[1] Butantan Inst, Special Lab Pain & Signaling, Sao Paulo - Brazil
[2] Butantan Inst, Lab Pathophysiol, Av Vital Brazil 1500, BR-05503900 Sao Paulo - Brazil
[3] Univ Sao Paulo, Inst Biomed Sci, Dept Pharmacol, Sao Paulo - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: Experimental Cell Research; v. 382, n. 2-3 SEP 15 2019.
Citações Web of Science: 0
Resumo

Advanced glycation end-products (AGEs) are proteins/lipids that are glycated upon sugar exposure and are often increased during inflammatory diseases such as osteoarthritis and neurodegenerative disorders. Here, we developed an extracellular matrix (ECM) using glycated type I collagen (ECM-GC), which produced similar levels of AGEs to those detected in the sera of arthritic mice. In order to determine whether AGEs were sufficient to stimulate sensory neurons, dorsal root ganglia (DRGs) cells were cultured on ECM-GC or ECM-NC-coated plates. ECM-GC or ECM-NC were favorable for DRG cells expansion. However, ECM-GC cultivated neurons displayed thinner F-actin filaments, rounded morphology, and reduced neuron interconnection compared to ECM-NC. In addition, ECM-GC did not affect RAGE expression levels in the neurons, although induced rapid p38, MAPK and ERK activation. Finally, ECM-GC stimulated the secretion of nitrite and TNF-alpha by DRG cells. Taken together, our in vitro glycated ECM model suitably mimics the in vivo microenvironment of inflammatory disorders and provides new insights into the role of ECM impairment as a nociceptive stimulus. (AU)

Processo FAPESP: 16/12128-0 - Mecanismos moleculares da dor na artrite: identificação de novos alvos para o desenvolvimento de fármacos
Beneficiário:Michelle Cristiane Búfalo
Linha de fomento: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 15/50040-4 - Rational approach for searching molecular targets involved in inflammatory events and cell survival
Beneficiário:Ana Marisa Chudzinski-Tavassi
Linha de fomento: Auxílio à Pesquisa - Programa Centros de Pesquisa em Engenharia
Processo FAPESP: 12/51241-5 - Efeito da crotoxina sobre eventos fundamentais do processo angiogênico avaliado em matriz extracelular bidimensional e tridimensional: estudos in vitro
Beneficiário:Sandra Coccuzzo Sampaio Vessoni
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 16/10886-4 - Ações de toxinas isoladas de diferentes venenos animais sobre a glicação de matriz extracelular no modelo de osteoartrite in vitro
Beneficiário:Maíra Estanislau Soares de Almeida
Linha de fomento: Bolsas no Brasil - Pós-Doutorado