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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Learning from transgenics: Advanced gene editing technologies should also bridge the gap with traditional genetic selection

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Arencibia, Ariel D. [1] ; D'Afonseca, Vivian [1, 2] ; Chakravarthi, Mohan [3, 4] ; Castiglione, Stefano [5]
Número total de Autores: 4
Afiliação do(s) autor(es):
[1] Univ Catolica Maule, Ctr Biotecnol Recursos Nat CenBio, Talca - Chile
[2] Univ Catolica Maule, Vicerrectoria Invest & Postgrad VRIP, Talca - Chile
[3] Univ Florida, Dept Agron, Gainesville, FL 32611 - USA
[4] Univ Fed Sao Carlos, Dept Genet & Evolucao, Sao Carlos, SP - Brazil
[5] Univ Salerno, Dipartimento Chim & Biol, Salerno - Italy
Número total de Afiliações: 5
Tipo de documento: Artigo de Revisão
Citações Web of Science: 0

We highlight the importance of the mixed genetic approaches (classical and currents) to improve the social perception related to the GMOs acceptance. We pointed out that CRISPR/Cas9 events could carry DNA variability/rearrangements related to somaclonal variations or epigenetic changes that are independent from the editing per se. The transformation of single cells, followed by plant regeneration, is used to generate modified plants, transgenic or genome editing (CRISPR/Cas9). The incidence of undesirable somadonal variations and/or epigenetic changes that might have occurred during in vitro multiplication and regeneration processes, must be carefully analyzed in replicates in field trials. One remarkable challenge is related to the time lapse that selects the modified elite genotypes, because these strategies may spend a variable amount of time before the results are commercialized, where in all the cases it should be take into account the genotype x environment interactions. Furthermore, this combination of techniques can create an encouraging bridge between the public opinion and the community of geneticists who are concerned with plant genetic improvement. In this context, either transgenesis or genomic editing strategies become complementary modern tools to facing the challenges of plant genetic improvement. Their applications will depend on case-by-case analysis, and when possible will necessary associate them to the schemes and bases of classic plant genetic improvement. (C) 2019 Pontificia Universidad Catolica de Valparaiso. Production and hosting by Elsevier B.V. All rights reserved. (AU)

Processo FAPESP: 18/11544-5 - Inserção dirigida de cistatinas em locus pré-caracterizados de cana-de-açúcar via CRISPR/Cas9
Beneficiário:Chakravarthi Mohan
Linha de fomento: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado