Busca avançada
Ano de início
Entree
(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

The C-Terminal Domains SnRK2 Box and ABA Box Have a Role in Sugarcane SnRK2s Auto-Activation and Activity

Texto completo
Autor(es):
Righetto, Germanna Lima [1] ; Sriranganadane, Dev [2, 3] ; Halabelian, Levon [4] ; Chiodi, Carla G. [2, 3] ; Elkins, Jonathan M. [5, 3] ; Massirer, Katlin B. [2, 3] ; Gileadi, Opher [5] ; Menossi, Marcelo [1] ; Counago, Rafael M. [2, 3]
Número total de Autores: 9
Afiliação do(s) autor(es):
[1] Univ Estadual Campinas, Funct Genome Lab, Dept Genet Evolut & Bioagents, Inst Biol, Campinas, SP - Brazil
[2] Univ Estadual Campinas UNICAMP, Ctr Quim Med CQMED, CBMEG, Campinas, SP - Brazil
[3] Univ Estadual Campinas, Struct Genom Consortium, Dept Genet & Evolucao, Inst Biol, Campinas, SP - Brazil
[4] MaRS Ctr, Struct Genom Consortium, Toronto, ON - Canada
[5] Univ Oxford, Nuffield Dept Med, Struct Genom Consortium, Oxford - England
Número total de Afiliações: 5
Tipo de documento: Artigo Científico
Fonte: FRONTIERS IN PLANT SCIENCE; v. 10, SEP 17 2019.
Citações Web of Science: 0
Resumo

Resistance to drought stress is fundamental to plant survival and development. Abscisic acid (ABA) is one of the major hormones involved in different types of abiotic and biotic stress responses. ABA intracellular signaling has been extensively explored in Arabidopsis thaliana and occurs via a phosphorylation cascade mediated by three related protein kinases, denominated SnRK2s (SNF1-related protein kinases). However, the role of ABA signaling and the biochemistry of SnRK2 in crop plants remains underexplored. Considering the importance of the ABA hormone in abiotic stress tolerance, here we investigated the regulatory mechanism of sugarcane SnRK2s-known as stress/ABA-activated protein kinases (SAPKs). The crystal structure of ScSAPK10 revealed the characteristic SnRK2 family architecture, in which the regulatory SnRK2 box interacts with the kinase domain alpha C helix. To study sugarcane SnRK2 regulation, we produced a series of mutants for the protein regulatory domains SnRK2 box and ABA box. Mutations in ScSAPK8 SnRK2 box aimed at perturbing its interaction with the protein kinase domain reduced protein kinase activity in vitro. On the other hand, mutations to ScSAPK ABA box did not impact protein kinase activity but did alter the protein autophosphorylation pattern. Taken together, our results demonstrate that both SnRK2 and ABA boxes might play a role in sugarcane SnRK2 function. (AU)

Processo FAPESP: 13/50724-5 - Centro de Biologia Química de Proteínas Quinases: alavancando desenvolvimento de fármacos através de pesquisa de acesso aberto
Beneficiário:Paulo Arruda
Linha de fomento: Auxílio à Pesquisa - Parceria para Inovação Tecnológica - PITE