| Texto completo | |
| Autor(es): |
Rossato, Luana
[1]
;
dos Santos, Suelen Silvana
[1]
;
Ferreira, Lucas Goncalves
[1]
;
de Almeida, Sandro Rogerio
[1]
Número total de Autores: 4
|
| Afiliação do(s) autor(es): | [1] Univ Sao Paulo, Fac Ciencias Farmaceut, Dept Anal Clin & Toxicol, Sao Paulo - Brazil
Número total de Afiliações: 1
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Medical Mycology; v. 57, n. 4, p. 489-495, JUN 2019. |
| Citações Web of Science: | 1 |
| Resumo | |
Here we investigated the importance of Toll-like receptor 4 (TLR-4) in innate immune response to Sporothrix brasiliensis, a virulent fungus of Sporothrix spp. In vitro assays, using C57Bl/6 (wild type {[}WT]) bone marrow-derived macrophages (BMDMs), and TLR-4 knockout (TLR-4(-/-)) showed that the absence of TLR-4 resulted in impaired phagocytosis and lower levels of tumor necrosis factor alpha (TNF-alpha), interleukin (IL)-6, and nitric oxide. In vivo assays were also performed, and the mice (WT and TLR-4(-/-)) were intraperitoneally infected with S. brasiliensis yeast ATCC MyA-4831 and euthanized on days 7, 14, and 28 postinfection, with the following parameters evaluated: fungal burden in liver, spleen, kidney, and brain, and the production of cytokines interferon gamma (IFN-alpha), TNF-alpha, IL-2, IL-4, IL-6, and IL-10. The results demonstrate the macrophages dependency on TLR-4 for inflammatory activation and in the absence of TLR-4 during experimental S. brasiliensis infection enhanced dissemination occurred after 14 and 28 days. These data show that TLR-4 signals are important for the recognition of S. brasiliensis by macrophages, and their absence promotes the persistence of the infection. (AU) | |
| Processo FAPESP: | 13/19213-4 - Estudos de interação de macrófagos frente à espécie Sporothrix brasiliensis. |
| Beneficiário: | Luana Rossato |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |