Bacterial communities associated with anthracnose symptomatic and asymptomatic leaves of guarana, an endogenous tropical crop, and their pathogen antagonistic effects

Autor(es)

Bonatelli, Maria Leticia ^[1] ; Tsui, Sarina ^[1] ; Batista, Bruna Durante ^[1] ; Dourado, Manuella Nobrega ^[2] ; Kitajima, Elliot Watanabe ^[3] ; Andreote, Fernando Dini ^[4] ; Pylro, Victor Satler ^[4] ; Pereira, Jose Odair ^[5] ; de Azevedo, Joao Lucio ^{[1, 5]} ; Quecine, Maria Carolina ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Luiz de Queiroz Coll Agr, Dept Genet, Piracicaba, SP - Brazil

^[2] Univ Sao Paulo, Dept Microbiol, Sao Paulo, SP - Brazil

^[3] Univ Sao Paulo, Dept Plant Pathol & Nematol, Luiz de Queiroz Coll Agr, Piracicaba, SP - Brazil

^[4] Univ Sao Paulo, Dept Soil Sci, Luiz de Queiroz Coll Agr, Piracicaba, SP - Brazil

^[5] Univ Fed Amazonas, UFAM, Manaus, Amazonas - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Archives of Microbiology; v. 201, n. 8, p. 1061-1073, OCT 2019.
• Citações Web of Science: 0

Resumo

Plants are colonized by diverse microorganisms that can substantially impact their health and growth. Understanding bacterial diversity and the relationships between bacteria and phytopathogens may be key to finding effective biocontrol agents. We evaluated the bacterial community associated with anthracnose symptomatic and asymptomatic leaves of guarana, a typical tropical crop. Bacterial communities were assessed through culture-independent techniques based on extensive 16S rRNA sequencing, and cultured bacterial strains were evaluated for their ability to inhibit the growth of Colletotrichum sp. as well as for enzyme and siderophore production. The culture-independent method revealed that Proteobacteria was the most abundant phylum, but many sequences were unclassified. The emergence of anthracnose disease did not significantly affect the bacterial community, but the abundance of the genera Acinetobacter, Pseudomonas and Klebsiella were significantly higher in the symptomatic leaves. In vitro growth of Colletotrichum sp. was inhibited by 11.38% of the cultured bacterial strains, and bacteria with the highest inhibition rates were isolated from symptomatic leaves, while asymptomatic leaves hosted significantly more bacteria that produced amylase and polygalacturonase. The bacterial isolate Bacillus sp. EpD2-5 demonstrated the highest inhibition rate against Colletotrichum sp., whereas the isolates EpD2-12 and FD5-12 from the same genus also had high inhibition rates. These isolates were also able to produce several hydrolytic enzymes and siderophores, indicating that they may be good candidates for the biocontrol of anthracnose. Our work demonstrated the importance of using a polyphasic approach to study microbial communities from plant diseases, and future work should focus on elucidating the roles of culture-independent bacterial communities in guarana anthracnose disease. (AU)

• Processo FAPESP: 09/53376-2 - Microrganismos associados ao guaranazeiro com potencial biotecnológico e agrícola, especialmente visando o controle da antracnose
• Beneficiário: Elliot Watanabe Kitajima
• Linha de fomento: Auxílio à Pesquisa - Regular
• Processo FAPESP: 11/05204-8 - Promoção de crescimento vegetal por rizobactérias do guaranazeiro
• Beneficiário: Bruna Durante Batista
• Linha de fomento: Bolsas no Brasil - Mestrado
• Processo FAPESP: 10/14382-4 - Microrganismos endofíticos cultiváveis e não cultiváveis do guaranazeiro e o controle da antracnose
• Beneficiário: Maria Leticia Bonatelli
• Linha de fomento: Bolsas no Brasil - Mestrado
• Processo FAPESP: 10/15192-4 - Estudo da diversidade de bactérias endofíticas cultiváveis associadas ao guaranazeiro [Paullinia cupana (Mart.) ducke.] visando o controle de antracnose
• Beneficiário: Sarina Tsui
• Linha de fomento: Bolsas no Brasil - Iniciação Científica