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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Detoxification, oxidative stress, and cytogenotoxicity of crack cocaine in the brown mussel Perna perna

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Autor(es):
Barbosa Ortega, Andressa dos Santos [1] ; Maranho, Luciane Alves [2, 1] ; Nobre, Caio Rodrigues [3] ; Moreno, Beatriz Barbosa [1] ; Guimaraes, Rafael Sole [1] ; Lebre, Daniel Temponi [4] ; de Souza Abessa, Denis Moledo [3] ; Ribeiro, Daniel Araki [5] ; Seabra Pereira, Camilo Dias [2, 1]
Número total de Autores: 9
Afiliação do(s) autor(es):
[1] Univ Fed Sao Paulo, Dept Ciencias Mar, Rua Maria Maximo 168, BR-11030100 Santos - Brazil
[2] Univ Santa Cecilia, Lab Ecotoxicol, Rua Oswaldo Cruz 266, BR-11045907 Santos - Brazil
[3] Univ Estadual Paulista, Inst Biociencias, Campus Litoral Paulista, Infante Dom Henrique S-N, BR-11330900 Sao Vicente - Brazil
[4] CIETEC IPEN, CEMSA Ctr Espectrometria Massas Aplicada, Av Prof Lineu Prestes 2242, Salas 112 & 113, BR-05508000 Sao Paulo - Brazil
[5] Univ Fed Sao Paulo, Dept Biociencias, Av Ana Costa 95, BR-11060001 Santos - Brazil
Número total de Afiliações: 5
Tipo de documento: Artigo Científico
Fonte: Environmental Science and Pollution Research; v. 26, n. 27, SI, p. 27569-27578, SEP 2019.
Citações Web of Science: 4
Resumo

The presence of cocaine and its metabolites and by-products has been identified in different aquatic matrices, making crack cocaine the target of recent studies. The aim of this study was to evaluate the sublethal effects of crack on the brown mussel Perna perna. Mussels were exposed to three concentrations of crack cocaine (0.5, 5.0, and 50.0 mu g L-1) for 168 h. Gills, digestive glands, and hemolymph were extracted and analyzed after three different exposure times using a suite of biomarkers (EROD, DBF, GST, GPX, LPO, DNA damage, ChE, and lysosomal membrane stability {[}LMS]). After 48 and 96 h of exposure, EROD, DBF, GST, GPX activities and DNA strand breaks in the gills increased significantly after 48 and 96 h of exposure. Alterations in LMS were also observed in the mussels exposed to all crack concentrations after 96 and 168 h. Our results demonstrated that crack cocaine is metabolized by CYP-like and GST activities in the gills. GPX was not able to prevent primary genetic damage, and cytotoxic effects in the hemocytes were also observed in a dose- and time-dependent response. Our study shows that the introduction of illicit drugs into coastal ecosystems must be considered a threat to marine organisms. (AU)

Processo FAPESP: 15/17329-0 - Estudo ecotoxicológico e avaliação do risco ambiental de drogas ilícitas em ecossistemas marinhos
Beneficiário:Camilo Dias Seabra Pereira
Linha de fomento: Auxílio à Pesquisa - Regular