Busca avançada
Ano de início
Entree
(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

The Absence of Pineal Melatonin Abolishes the Daily Rhythm of Tph1 (Tryptophan Hydroxylase 1), Asmt (Acetylserotonin O-Methyltransferase), and Aanat (Aralkylamine N-Acetyltransferase) mRNA Expressions in Rat Testes

Texto completo
Autor(es):
Coelho, L. A. [1] ; Andrade-Silva, J. [2] ; Motta-Teixeira, L. C. [2] ; Amaral, F. G. [3] ; Reiter, R. J. [4] ; Cipolla-Neto, J. [2]
Número total de Autores: 6
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Fac Anim Sci & Food Engn FZEA, Dept Vet Med, Av Duque Caxias Norte 225, BR-13635900 Pirassununga, SP - Brazil
[2] Univ Sao Paulo, Inst Biomed Sci ICB, Dept Physiol & Biophys, Sao Paulo, SP - Brazil
[3] Fed Univ Sao Paulo UNIFESP, Dept Physiol, Sao Paulo, SP - Brazil
[4] Univ Texas Hlth Sci Ctr San Antonio, Dept Cellular & Struct Biol, San Antonio, TX 78229 - USA
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: Molecular Neurobiology; v. 56, n. 11, p. 7800-7809, NOV 2019.
Citações Web of Science: 0
Resumo

This study examined the effects of pinealectomy in Wistar rats and melatonin replacement therapy on the daily mRNA expression of melatonin (Tph1, Aanat, Asmt, Mt1, Mt2, and Ror alpha), and steroidogenic (Star, 17 beta hsd3, and Lhr) related genes as well as clock genes (Rev-erb alpha, Bmal1, Per1, Per2, Cry1, and Cry2) in testes. The testes of control animals express the Tph1, Aanat, and Asmt and Per2 genes with 24-h rhythms in mRNA, reaching the maximal values during the dark phase. Pinealectomy abolished and melatonin treatment restored the 24-h rhythmicity. Daytime differences in mRNA expression were significant for Star, Lhr, Mt1, Mt2, Ror alpha, Rev-erb alpha, Bmal1, Cry1, and Cry2 genes in testes of control rats. Conversely, 17 beta hsd3 and Per1 mRNA expression did not show a daytime difference in testes of control animals. Pinealectomy abolished the peak time of Mt1 and Mt2 mRNA expression, phase shifted the peak time of Star, Ror alpha, Rev-erb alpha, Bmal1, and Cry2 mRNA expression, downregulated the 24-h Lhr mRNA expression, and inverted the peak time of Per1, Per2, and Cry1 mRNA expression to the light phase. The melatonin replacement therapy completely restored the control levels of Lhr, Rev-erb alpha, and Per1 mRNA expression patterns, partially restored the daily control of Star, Mt2, Ror alpha, Bmal1, Cry1, and Cry2 mRNA expression but did not re-establish the daily control of Mt1 mRNA expression. This suggests that the daily mRNA expression of these genes is probably driven by pineal melatonin and melatonin treatment restores (partially or completely) the daily control of gene expression patterns. (AU)

Processo FAPESP: 14/50457-0 - Melatonina e a regulação do metabolismo energético: estudos básicos, clínicos e epidemiológicos
Beneficiário:José Cipolla Neto
Linha de fomento: Auxílio à Pesquisa - Temático
Processo FAPESP: 14/17830-9 - Papel da melatonina em alguns aspectos da maturação in vitro dos ovócitos de mamíferos
Beneficiário:Lia de Alencar Coelho
Linha de fomento: Auxílio à Pesquisa - Regular