Fecal bile acid profile after Roux-en-Y gastric bypass and its association with the remission of type 2 diabetes in obese women: A preliminary study

Autor(es)

Cardinelli, Camila de Siqueira ^[1] ; Torrinhas, Raquel Susana ^[1] ; Sala, Priscila ^[1] ; Pudenzi, Marcos Albieri ^[2] ; Angolini, Celio Fernando F. ^[2] ; da Silva, Mariane Marques ^[1] ; Machado, Natasha Mendonca ^[1] ; Ravacci, Graziela ^[1] ; Eberlin, Marcos N. ^[2] ; Waitzberg, Dan L. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Med, Lab Nutr & Surg Metab Digest Tract, Dept Gastroenterol, Metanutri Lim 35, Sao Paulo, SP - Brazil

^[2] State Univ Campinas UNICAMP, ThoMSon Mass Spectrometry Lab, Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Clinical Nutrition; v. 38, n. 6, p. 2906-2912, DEC 2019.
• Citações Web of Science: 1

Resumo

Objective: To assess the influence of Roux-en-Y gastric by-pass (RYGB) on fecal bile acid (BA) profile and its relationship with postoperative remission of type 2 diabetes (T2D). Methods: Fecal samples were collected 3 and 12 months after RYGB from diabetic obese women who were responsive (n = 12) and non-responsive (n = 8) to postoperative remission of T2D. Fecal BA profile was accessed by liquid chromatography coupled to tandem mass spectrometry in a targeted approach. Results: Relative to pre-operative levels, a total of 10 fecal BA profiles decreased after RYGB (ANOVA, p <= 0.05) with significant fold-changes for glycochenodeoxycholic, glycocholic, taurocholic, and taurochenodeoxycholic acids at 3-months postoperatively, and for glycochenodeoxycholic, glycocholic and taurocholic acids at 12 months postoperatively (Benjamini-Hochberg, p < 0.05). Postoperative changes in fecal BA were different between responsive and non-responsive women, with a significant reduction in more sub-fractions of BA in responsive women than in non-responsive women, and a marked difference in the temporal behavior of cholic acid (CA) and chenodeoxycholic acid (CDCA), thus reflecting changes in CA/CDCA ratio, and tauroursodeoxycolic (TUDCA) levels between these responsiveness groups (ANOVA, p <= 0.05). Conclusion: RYGB induces a marked reduction in the concentration of fecal BA, which is heterogeneous according to T2D responsiveness. (C) 2019 Elsevier Ltd and European Society for Clinical Nutrition and Metabolism. All rights reserved. (AU)

• Processo FAPESP: 14/05220-1 - Metabolômica da água fecal de pacientes obesos com Diabetes tipo 2 antes e após gastroplastia redutora em Y-Roux (GRYR)
• Beneficiário: Camila de Siqueira Cardinelli
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 11/09612-3 - Alterações na expressão gênica do tecido gástrico e intestinal de portadores de Diabetes melito tipo 2 submetidos à gastroplastia redutora a Y-Roux
• Beneficiário: Dan Linetzky Waitzberg
• Modalidade de apoio: Auxílio à Pesquisa - Temático