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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Inactivation kinetics and lethal dose analysis of antimicrobial blue light and photodynamic therapy

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Sabino, Caetano P. [1, 2] ; Wainwright, Mark [3] ; dos Anjos, Carolina [4] ; Sellera, Fabio P. [4] ; Baptista, Mauricio S. [5] ; Lincopan, Nilton [6, 1] ; Ribeiro, Martha S. [7]
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Sch Pharmaceut Sci, Dept Clin Anal, Sao Paulo, SP - Brazil
[2] BioLambda, Sci & Commercial LTD, Sao Paulo, SP - Brazil
[3] Liverpool John Moores Univ, Sch Pharm & Biomol Sci, Liverpool, Merseyside - England
[4] Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Internal Med, Sao Paulo, SP - Brazil
[5] Univ Sao Paulo, Inst Chem, Dept Biochem, Sao Paulo, SP - Brazil
[6] Univ Sao Paulo, Inst Biomed Sci, Dept Microbiol, Sao Paulo, SP - Brazil
[7] Natl Commiss Nucl Energy, Ctr Lasers & Applicat, Nucl & Energy Res Inst, Sao Paulo, SP - Brazil
Número total de Afiliações: 7
Tipo de documento: Artigo Científico
Fonte: Photodiagnosis and Photodynamic Therapy; v. 28, p. 186-191, DEC 2019.
Citações Web of Science: 1

Background: Antimicrobial Photodynamic therapy (A-PDT) has been used to treat infections. Currently, microbial inactivation data is reported presenting survival fraction averages and standard errors as discrete points instead of a continuous curve of inactivation kinetics. Standardization of this approach would allow clinical protocols to be introduced globally, instead of the piecemeal situation which currently applies. Methods: To this end, we used a power-law function to fit inactivation kinetics and directly report values of lethal doses (LD) and a tolerance factor (T) that informs if inactivation rate varies along the irradiation procedure. A deduced formula was also tested to predict LB for any given survival fraction value. We analyzed the photoantimicrobial effect caused by red light activation of methylene blue (MB-APDT) and by blue light (BL) activation of endogenous microbial pigments against 5 clinically relevant pathogens. Results: Following MB- APDT, Escherichia coli and Staphylococcus aureus cells become increasingly more tolerant to inactivation along the irradiation process (T < 1). Klebsiella pneumoniae presents opposite behavior, i.e., more inactivation is observed towards the end of the process (T > 1). P. aeruginosa and Candida albicans present constant inactivation rate (T(similar to)1). In contrast, all bacterial species presented similar behavior during inactivation caused by BL, i.e., continuously becoming more sensitive to blue light exposure (T > 1). Conclusion: The power-law function successfully fit all experimental data. Our proposed method precisely predicted LD and T values. We expect that these analytical models may contribute to more standardized methods for comparisons of photodynamic inactivation efficiencies. (AU)

Processo FAPESP: 16/08593-9 - Pan-resistoma de Klebsiella pneumoniae e Escherichia coli produtoras de beta-lactamases (KPC-2, CTX-M-8, CTX-M-15) endêmicas no Brasil
Beneficiário:Nilton Erbet Lincopan Huenuman
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 13/07937-8 - Redoxoma
Beneficiário:Ohara Augusto
Linha de fomento: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
Processo FAPESP: 17/22406-0 - Desenvolvimento de equipamento inteligente para aplicação clínica de fototerapias com ajuste dosimétrico automático e pagamento sob demanda
Beneficiário:Caetano Padial Sabino
Linha de fomento: Auxílio à Pesquisa - Pesquisa Inovativa em Pequenas Empresas - PIPE