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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Quantitative Subcellular Proteomics of the Orbitofrontal Cortex of Schizophrenia Patients

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Autor(es):
Velasquez, Erika [1] ; Martins-de-Souza, Daniel [2, 3, 4] ; Velasquez, Ingrid [5] ; Alves Carneiro, Gabriel Reis [1, 6] ; Schmitt, Andrea [7] ; Falkai, Peter [7] ; Domont, Gilberto B. [1] ; Nogueira, Fabio C. S. [1, 6]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Univ Fed Rio de Janeiro, Inst Chem, Dept Biochem, Prote Unit, BR-21941909 Rio De Janeiro - Brazil
[2] Univ Campinas UNICAMP, Inst Biol, Dept Biochem, Lab Neuroprote, BR-13083970 Campinas, SP - Brazil
[3] Univ Estadual Campinas, EMRC, BR-13083887 Campinas, SP - Brazil
[4] Conselho Nacl Desenvolvimento Cient & Tecnol CNPq, Inst Nacl Biomarcadores Neuropsiquiatria INBION, Sao Paulo, SP - Brazil
[5] Univ Carabobo, Naguanagua 2005, Carabobo - Venezuela
[6] Univ Fed Rio de Janeiro, Inst Chem, LADETEC, Lab Prote, BR-21941598 Rio De Janeiro - Brazil
[7] Ludwig Maximilian Univ Munich LMU, Dept Psychiat & Psychotherapy, D-80539 Munich - Germany
Número total de Afiliações: 7
Tipo de documento: Artigo Científico
Fonte: JOURNAL OF PROTEOME RESEARCH; v. 18, n. 12, SI, p. 4240-4253, DEC 2019.
Citações Web of Science: 1
Resumo

Schizophrenia is a chronic disease characterized by the impairment of mental functions with a marked social dysfunction. A quantitative proteomic approach using iTRAQ labeling and SRM, applied to the characterization of mitochondria (MIT), crude nuclear fraction (NUC), and cytoplasm (CYT), can allow the observation of dynamic changes in cell compartments providing valuable insights concerning schizophrenia physiopathology. Mass spectrometry analyses of the orbitofrontal cortex from 12 schizophrenia patients and 8 healthy controls identified 655 protein groups in the MIT fraction, 1500 in NUC, and 1591 in CYT. We found 166 groups of proteins dysregulated among all enriched cellular fractions. Through the quantitative proteomic analysis, we detect as the main biological pathways those related to calcium and glutamate imbalance, cell signaling disruption of CREB activation, axon guidance, and proteins involved in the activation of NF-kB signaling along with the increase of complement protein C3. Based on our data analysis, we suggest the activation of NF-kB as a possible pathway that links the deregulation of glutamate, calcium, apoptosis, and the activation of the immune system in schizophrenia patients. All MS data are available in the ProteomeXchange Repository under the identifier PXD015356 and PXD014350. (AU)

Processo FAPESP: 19/00098-7 - EMU concedido no processo 2017/25588-1: cromatógrafo Acquity UPLC I-Class
Beneficiário:Daniel Martins-de-Souza
Linha de fomento: Auxílio à Pesquisa - Programa Equipamentos Multiusuários
Processo FAPESP: 17/25588-1 - Da compreensão básica a biomarcadores clínicos para a esquizofrenia: um estudo multidisciplinar centrado na neuroproteômica
Beneficiário:Daniel Martins-de-Souza
Linha de fomento: Auxílio à Pesquisa - Temático