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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Cell wall hydrolases act in concert during aerenchyma development in sugarcane roots

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Autor(es):
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Grandis, Adriana [1] ; Leite, Debora C. C. [1] ; Tavares, Eveline Q. P. [1] ; Arenque-Musa, Bruna C. [1] ; Gaiarsa, Jonas W. [2] ; Martins, Marina C. M. [1] ; De Souza, Amanda P. [1, 3] ; Gomez, Leonardo D. [4] ; Fabbri, Claudia [5] ; Mattei, Benedetta [5, 6] ; Buckeridge, Marcos S. [1]
Número total de Autores: 11
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Inst Biosci, Dept Bot, Lab Plant Physiol Ecol LAFIECO, Rua Matao 277, Sao Paulo, SP - Brazil
[2] Univ Sao Paulo, Inst Biosci, Dept Bot, GaTE Lab, Sao Paulo, SP - Brazil
[3] Univ Illinois, Carl R Woese Inst Genom Biol, 1206 West Gregory Dr, Urbana, IL 61801 - USA
[4] Univ York, Dept Biol, Ctr Novel Agr Prod, York, N Yorkshire - England
[5] Univ Rome Sapienza, Dept Biol & Biotechnol C Darwin, Rome - Italy
[6] Univ Aquila, Dept Life Hlth & Environm Sci, Laquila - Italy
Número total de Afiliações: 6
Tipo de documento: Artigo Científico
Fonte: ANNALS OF BOTANY; v. 124, n. 6, SI, p. 1067-1089, NOV 4 2019.
Citações Web of Science: 1
Resumo

Background and Aims Cell wall disassembly occurs naturally in plants by the action of several glycosyl-hydrolases during different developmental processes such as lysigenous and constitutive aerenchyma formation in sugarcane roots. Wall degradation has been reported in aerenchyma development in different species, but little is known about the action of glycosyl-hydrolases in this process. Methods In this work, gene expression, protein levels and enzymatic activity of cell wall hydrolases were assessed. Since aerenchyma formation is constitutive in sugarcane roots, they were assessed in segments corresponding to the first 5 cm from the root tip where aerenchyma develops. Key Results Our results indicate that the wall degradation starts with a partial attack on pectins (by acetyl esterases, endopolygalacturonases, beta-galactosidases and alpha-arabinofuranosidases) followed by the action of beta-glucan-/callose-hydrolysing enzymes. At the same time, there are modifications in arabinoxylan (by alpha-arabinofuranosidases), xyloglucan (by XTH), xyloglucan-cellulose interactions (by expansins) and partial hydrolysis of cellulose. Saccharification revealed that access to the cell wall varies among segments, consistent with an increase in recalcitrance and composite formation during aerenchyma development. Conclusion Our findings corroborate the hypothesis that hydrolases are synchronically synthesized, leading to cell wall modifications that are modulated by the fine structure of cell wall polymers during aerenchyma formation in the cortex of sugarcane roots. (AU)

Processo FAPESP: 10/17070-3 - Determinação das enzimas de degradação da parede celular durante a formação do aerênquima em raízes de cana de açúcar
Beneficiário:Adriana Grandis
Linha de fomento: Bolsas no Brasil - Doutorado
Processo FAPESP: 10/17104-5 - Degradação da parede celular em raízes de cana-de-açúcar
Beneficiário:Marcos Silveira Buckeridge
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 14/50884-5 - INCT 2014: Instituto Nacional de Ciência e Tecnologia do Bioetanol
Beneficiário:Marcos Silveira Buckeridge
Linha de fomento: Auxílio à Pesquisa - Temático
Processo FAPESP: 09/52840-7 - Centro de Processos Biológicos e Industriais para Biocombustíveis - CeProBIO
Beneficiário:Igor Polikarpov
Linha de fomento: Auxílio à Pesquisa - Programa BIOEN - Temático
Processo FAPESP: 08/57908-6 - Instituto Nacional de Biotecnologia para o Bioetanol
Beneficiário:Marcos Silveira Buckeridge
Linha de fomento: Auxílio à Pesquisa - Programa BIOEN - Temático