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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

IgG from atopic dermatitis patients induces non-atopic infant thymic invariant natural killer T (iNKT) cells to produce IL-4, IL-17, and IL-10

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Autor(es):
Santos, Ludimila S. [1, 2] ; Sgnotto, Fabio da Ressureicao [3] ; Sousa, Thamires R. [2] ; Orfali, Raquel L. [2] ; Aoki, Valeria [2] ; da Silva Duarte, Alberto Jose [4, 2] ; Victor, Jefferson R. [1, 2]
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] Laureate Int Univ, FMU, Div Environm Hlth, Sao Paulo - Brazil
[2] Univ Sao Paulo, Sch Med, Div Clin Dermatol, Lab Med Invest LIM 56, Ave Dr En eas de Carvalho Aguiar 500, 3rd Floor, BR-05403000 Sao Paulo - Brazil
[3] Univ Sao Paulo, Div Hematol, Sch Med, Sao Paulo - Brazil
[4] Univ Sao Paulo, Sch Med, Div Pathol, Sao Paulo - Brazil
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: INTERNATIONAL JOURNAL OF DERMATOLOGY; OCT 2019.
Citações Web of Science: 0
Resumo

Background Atopic dermatitis (AD) pathogenesis still needs to be elucidated, but invariant natural killer T (iNKT) cell involvement was already described by several groups. Our group has demonstrated that IgG antibodies purified from AD patients can modulate cytokine production by thymic T cells. Here we aimed to investigate if IgG from AD patients can modulate infant non-atopic thymic iNKT cells cytokine production in order to collaborate with the elucidation of AD development in infancy. Methods Thymic tissues were obtained from children from non-atopic mothers, and IgG was purified from AD patients diagnosed as moderate or severe and, as controls, from subjects clinically classified as non-atopic individuals. PBMCs from non-atopic individuals were also used in this study. Results Our results demonstrated that IgG from AD patients could induce non-atopic children thymic iNKT cells to produce higher levels of intracellular IL-4, IL-10, and IL-17 when compared to all control conditions. No effect was observed in non-atopic adults peripheral iNKT. We also observed that IgG from AD patients induces an increase in the expression of CD4 and Ror gamma t transcription factor in non-atopic children thymic iNKT cells compared to the condition of all controls. Conclusions These observations suggest that IgG from AD patients can induce a cytokine profile by thymic iNKT cells from non-atopic infants compatible with the observations in AD development, which can collaborate with the elucidation of AD pathogenesis. (AU)

Processo FAPESP: 18/05181-7 - Avaliação translacional do efeito modulador e/ou regulador dos anticorpos IgG na maturação de linfócitos TCD4, TCD8, nTreg, Tgammadelta e B intra-tímicos de neonatos.
Beneficiário:Alberto José da Silva Duarte
Linha de fomento: Auxílio à Pesquisa - Regular