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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Role of testosterone and androgen receptor in periodontal disease progression in female rats

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Autor(es):
Steffens, Joao Paulo [1] ; Valenga, Henrique Meister [1] ; Leal Santana, Luis Carlos [2] ; da Costa Albaricci, Maria Carolina [2] ; Kantarci, Alpdogan [3] ; Spolidorio, Luis Carlos [2]
Número total de Autores: 6
Afiliação do(s) autor(es):
[1] Univ Fed Parana UFPR, Dept Stomatol, Curitiba, PR - Brazil
[2] Univ Estadual Paulista UNESP, Sch Dent Araraquara, Dept Physiol & Pathol, Araraquara, SP - Brazil
[3] Forsyth Inst, Dept Appl Oral Sci, Cambridge, MA - USA
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: Journal of Periodontology; AUG 2019.
Citações Web of Science: 0
Resumo

Background Sex hormone therapy has strict recommendations in the treatment of postmenopausal symptoms, in which testosterone (TES) replacement may play a potential role. However, it remains unclear whether TES affects the course of chronic inflammation and alveolar bone loss in females. Herein, we investigated the role of androgen receptor and TES on the inflammatory response and alveolar bone resorption associated with ligature-induced periodontal disease in female rats. Methods Fifty female Holtzman rats were divided in five groups (n = 10/group): androgen receptor antagonist (flutamide); estrogen receptor antagonist (fulvestrant); TES supplementation; aromatase inhibitor (anastrozole); and TES plus anastrozole. Periodontitis was induced by ligatures around the lower first molars for 2 weeks. Twenty animals (n = 10/group) were used as untreated ligated or non-ligated controls. Bone loss and the number of osteoclasts were measured through radiographic and immunohistochemical analysis, respectively. Inflammatory cytokines, chemokines and bone markers were measured by multiplex immunoassay and ELISA in serum samples and periodontal tissues. Results The blockage of androgen receptor significantly increased radiographic bone loss and tissue levels of IL-1 alpha (P <0.05), IL-1 beta (P <0.001) and IL-10 (P <0.01) compared with the periodontitis group. Testosterone supplementation significantly increased EGF levels in tissue samples, whereas when combined with aromatase inhibitor anastrozole significantly increased both EGF and VEGF (P <0.05). None of the treatment conditions significantly impacted the number of osteoclasts compared with the periodontitis group. Conclusions Androgen receptor activation is an important factor in the regulation of several inflammatory markers, and its blockage significantly increases bone loss. (AU)

Processo FAPESP: 13/07833-8 - Participação de andrógenos na regulação da resposta imunoinflamatória e metabolismo ósseo associados à saúde, doença e reparo periodontal em fêmeas
Beneficiário:João Paulo Steffens
Linha de fomento: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 13/23116-4 - Impacto de andrógenos na resposta inflamatória, osteoclastogênese e expressão de receptores para hormônios sexuais nos tecidos periodontais de ratas em condições de saúde, doença e reparo periodontal
Beneficiário:Maria Carolina da Costa Albaricci
Linha de fomento: Bolsas no Brasil - Iniciação Científica
Processo FAPESP: 13/12014-6 - Participação de andrógenos na regulação da resposta imunoinflamatória e metabolismo ósseo associados à saúde, doença e reparo periodontal em fêmeas
Beneficiário:Luis Carlos Spolidorio
Linha de fomento: Auxílio à Pesquisa - Regular