High similarity and high frequency of virulence genes among Salmonella Dublin strains isolated over a 33-year period in Brazil

Autor(es)

Vilela, Felipe Pinheiro ^[1] ; Rodrigues, Dalia dos Prazeres ^[2] ; Costa, Renata Garcia ^[2] ; Tiba Casas, Monique Ribeiro ^[3] ; Falcao, Juliana Pfrimer ^[1] ; Campioni, Fabio ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Anal Clin Toxicol & Bromatol, Fac Ciencias Farmaceut Ribeirao Preto, Av Caf S-N, BR-14040903 Ribeirao Preto, SP - Brazil

^[2] Fundacao Oswaldo Cruz, FIOCRUZ, Av Brasil 4365, BR-21040900 Rio De Janeiro, RJ - Brazil

^[3] Adolfo Lutz Inst, Ctr Bacteriol, Av Dr Arnaldo 351, BR-01255000 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Journal of Microbiology; v. 51, n. 2 NOV 2019.
• Citações Web of Science: 1

Resumo

Salmonella Dublin is a strongly adapted serovar that causes enteritis and/or systemic disease with high rates of mortality in cattle and occasionally infects humans. Despite the importance of this serovar, there is a lack of studies in Brazil. The aim of this study was to characterize the genetic diversity of 112 S. Dublin strains isolated from humans and animals in Brazil by CRISPR and CRISPR-MVLST and the relatedness among strains by MLST. In addition, the frequency of some important virulence genes was verified. The strains studied belonged to nine different sequence types, being all of them single- or double-locus variants of the ST10. CRISPR discriminated the strains into 69 subtypes with a similarity >= 84.4% and CRISPR-MVLST into 72 subtypes with a similarity >= 84.7%. The virulence genes ratB, lpfA, mgtC, avrA, sopB, sopE2, sifA, sseA, ssrA, csgA, fliC, and sinH were found in all the strains studied, while spvB, spvC, sodCl, rpoS, sipA, sipD, invA, and hilA were detected in >= 93.7% of the strains. In conclusion, the high similarity among the strains reinforces the clonal nature of the strains of this serovar that may have descended from a common ancestor that little differed over 33 years in Brazil. CRISPR and CRISPR-MVLST showed to be good alternatives to type S. Dublin strains. MLST suggested that S. Dublin strains from Brazil were phylogenetically related to strains from other parts of the globe. Moreover, the high frequency of virulence genes among the strains studied reinforces the capacity of S. Dublin to cause invasive diseases. (AU)

• Processo FAPESP: 17/05756-7 - Análise da invasão celular, sobrevivência em macrófagos e expressão dos genes st313-td, sopE2 e fliC em linhagens de Salmonella Dublin isoladas no Brasil
• Beneficiário: Felipe Pinheiro Vilela
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 19/06947-6 - Caracterização molecular da diversidade genotípica, perfil de resistência e potencial patogênico de linhagens de Salmonella infantis isoladas de fontes diversas no Brasil
• Beneficiário: Felipe Pinheiro Vilela
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 13/25191-3 - Análise fenotípica e sequenciamento do transcriptoma e genoma em linhagens de Salmonella Enteritidis isoladas no período pré e pós-pandemia no Brasil
• Beneficiário: Fábio Campioni
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 16/24716-3 - Sequenciamento do genoma, transcriptoma e análise fenotípica de linhagens de Campylobacter coli isoladas de diversas fontes no Brasil
• Beneficiário: Juliana Pfrimer Falcão
• Modalidade de apoio: Auxílio à Pesquisa - Regular