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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Laurdan as fluorescent probe to determinate the critical micelle temperature of polymers from Pluronic (R)-coated fluid phase liposomes

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Autor(es):
Calori, Italo Rodrigo [1, 2] ; Pazin, Wallance Moreira [3, 4] ; Brunaldi, Kellen [5] ; Pellosi, Diogo Silva [6] ; Caetano, Wilker [1] ; Tedesco, Antonio Claudio [2] ; Hioka, Noboru [1]
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] Univ Estadual Maringa, Dept Chem, Res Nucleus Photodynam Therapy, Av Colombo 5790, BR-97020900 Maringa, Parana - Brazil
[2] Univ Sao Paulo, Fac Philosophy Sci & Letters Ribeirao Preto, Biol & Photomed Res Grp, Dept Chem, Ctr Nanotechnol & Tissue Engn, BR-14040901 Ribeirao Preto, SP - Brazil
[3] Univ Sao Paulo, Fac Philosophy Sci & Letters Ribeirao Preto, Dept Phys, BR-14040901 Ribeirao Preto, SP - Brazil
[4] Sao Paulo State Univ UNESP, Sch Technol & Appl Sci, Dept Phys, Presidente Prudente, SP - Brazil
[5] Univ Estadual Maringa, Dept Physiol Sci, Av Colombo 5790, BR-97020900 Maringa, Parana - Brazil
[6] Univ Fed Sao Paulo, Dept Chem, Lab Hybrid Mat, Rua Sao Nicolau 210, BR-09913030 Diadema, SP - Brazil
Número total de Afiliações: 6
Tipo de documento: Artigo Científico
Fonte: JOURNAL OF MOLECULAR LIQUIDS; v. 294, NOV 15 2020.
Citações Web of Science: 0
Resumo

Future-generation liposomes for efficient drug-delivery systems may utilize multifunctional polymer coatings for stabilization and in order to take advantage of conferred properties such as stealth characteristics. In this scenario, understanding the dynamics of polymers coating liposomes versus polymers self-assembly as micelles is a critical issue. This paper describes a methodology to determinate the boundary between the presence of only liposomes and the coexistence of liposomes and micelles in mixed Pluronic (R)/fluid-phase lipid system, using Laurdan as a fluorescent probe. The temperature-mediated transition at the boundary region results in changes to the generalized polarization function of Laurdan, mainly due to the partition of Laurdan into micelles and increased exposure of the Laurdan probe to water at the liposome interfaces. The new methodology showed to be more sensitive than DLS and allows monitoring of the different organizations of polymers in polymer/lipid mixtures via a simple experimental technique. This new methodology should facilitate the development of smart-polymer-coated liposomes for drug delivery. Furthermore, its principle may be applied to the study of micelle-cell-membrane interactions in such applications. (C) 2019 Elsevier B.V. All rights reserved. (AU)

Processo FAPESP: 16/09633-4 - Mecanismo de ação do composto bioativo Artepillin C em sistemas miméticos de membranas e membranas citoplasmáticas de células tumorais
Beneficiário:Wallance Moreira Pazin
Linha de fomento: Bolsas no Brasil - Pós-Doutorado