The Supraoptic Nucleus of the Hypothalamus Modulates Autonomic, Neuroendocrine, and Behavioral Responses to Acute Restraint Stress in Rats

Autor(es)

Lopes-Azevedo, Silvana ^[1] ; Trindade Fortaleza, Eduardo Albino ^[1] ; Busnardo, Cristiane ^[1] ; Scopinho, America Augusto ^[1] ; Matthiesen, Melina ^[1] ; Antunes-Rodrigues, Jose ^[1] ; Aguiar Correa, Fernando Morgan ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Med Ribeirao Preto, Dept Pharmacol, Av Bandeirantes, BR-14049900 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Neuroendocrinology; v. 110, n. 1-2, p. 10-22, JAN 2020.
• Citações Web of Science: 1

Resumo

Aims: Acute restraint stress (RS) has been reported to cause neuronal activation in the supraoptic nucleus of the hypothalamus (SON). The aim of the study was to evaluate the role of SON on autonomic (mean arterial pressure {[}MAP], heart rate {[}HR], and tail temperature), neuroendocrine (corticosterone, oxytocin, and vasopressin plasma levels), and behavioral responses to RS. Methods: Guide cannulas were implanted bilaterally in the SON of male Wistar rats for microinjection of the unspecific synaptic blocker cobalt chloride (CoCl2, 1 mM) or vehicle (artificial cerebrospinal fluid, 100 nL). A catheter was introduced into the femoral artery for MAP and HR recording. Rats were subjected to RS, and it was studied the effect of microinjection of CoCl2 or vehicle into the SON on pressor and tachycardic responses, drop in tail temperature, plasma oxytocin, vasopressin, and corticosterone levels, and anxiogenic-like effect induced by RS. Results: SON pretreatment with CoCl2 reduced the RS-induced MAP and HR increase, without affecting the RS-evoked tail temperature decrease. Microinjection of CoCl2 into areas surrounding the SON did not affect RS-induced increase in MAP and HR, reinforcing the idea that SON influences RS-evoked cardiovascular responses. Also, SON pretreatment with CoCl2 reduced RS-induced increase in corticosterone and oxytocin, without affecting vasopressin plasma levels, suggesting its involvement in RS-induced neuroendocrine responses. Finally, the CoCl2 microinjection into SON inhibited the RS-caused delayed anxiogenic-like effect. Conclusion: The results indicate that SON is an important component of the neural pathway that controls autonomic, neuroendocrine, and behavioral responses induced by RS. (AU)

• Processo FAPESP: 14/22228-6 - Função do núcleo supra-óptico do hipotálamo nas respostas cardiovascular e neuroendócrina observadas em ratos submetidos ao estresse por restrição
• Beneficiário: Silvana Lopes Azevedo
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 13/13721-8 - Estudo do envolvimento do sistema endocanabinóide do córtex pré-frontal medial ventral sobre a ingestão alimentar de ratos.
• Beneficiário: América Scopinho Augusto
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado