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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Identification of Zinc Absorption Biomarkers in Muscle Tissue of Nile Tilapia Fed with Organic and Inorganic Sources of Zinc Using Metallomics Analysis

Texto completo
Autor(es):
de Lima, Paula Monteiro [1] ; Vieira, Jose Cavalcante Souza [2, 3] ; Cavecci-Mendonca, Bruna [1] ; Fleuri, Luciana Francisco [2] ; De Lima Leite, Aline [4] ; Buzalaf, Marilia Afonso Rabelo [4] ; Pezzato, Luiz Edivaldo [1] ; Braga, Camila Pereira [5] ; de Magalhaes Padilha, Pedro [2, 1]
Número total de Autores: 9
Afiliação do(s) autor(es):
[1] Sao Paulo State Univ UNESP, Sch Vet Med & Anim Sci, Botucatu, SP - Brazil
[2] Sao Paulo State Univ UNESP, Inst Biosci, Botucatu, SP - Brazil
[3] Fed Univ Mato Grosso Sul UFMS, Campo Grande, MS - Brazil
[4] Univ Sao Paulo, Biochem Dept, Bauru, SP - Brazil
[5] Univ Nebraska UNL, Biochem Dept, Lincoln, NE - USA
Número total de Afiliações: 5
Tipo de documento: Artigo Científico
Fonte: BIOLOGICAL TRACE ELEMENT RESEARCH; v. 194, n. 1, p. 259-272, MAR 2020.
Citações Web of Science: 0
Resumo

The development of metallomics techniques has allowed for metallomics analysis of biological systems, enabling a better understanding of the response mechanisms for different stimuli, their relationship to metallic species, and the characterization of biomarkers. In this study, a metallomics analysis of the muscle tissue of Nile tilapia was used to aid the understanding of the molecular mechanisms involved in zinc absorption in this fish species when fed organic and/or inorganic sources of zinc and to identify possible biomarkers for the absorption of this micromineral. To accomplish this, the fish were separated into three groups of 24 g, 74 g, and 85 g initial weights, and each group, respectively, was fed a zinc-free diet (control group, G1), a diet containing zinc found in organic sources (treatment 1, G2), and a diet containing zinc from an inorganic source (treatment 2, G3). Two-dimensional polyacrylamide (2D PAGE) gel electrophoresis was used to separate the proteins of the muscle tissue. Subsequently, the expression profiles of protein spots in the samples where zinc was applied in different concentrations were compared, using the software ImageMaster 2D Platinum version 7.0, to identify proteins that were differentially expressed. The identified proteins were then exposed to atomic absorption spectrometry in a graphite furnace to determine zinc mapping and were subsequently characterized via electrospray ionization tandem mass spectrometry (ESI-MS/MS). The metallomic analysis identified 15 proteins differentially expressed and associated with zinc, leading to the conclusion that three metal-binding proteins presented as possible biomarkers of zinc absorption in fish. (AU)

Processo FAPESP: 10/51332-5 - Desenvolvimento de métodos analíticos para estudo metalômico do mercúrio em peixes coletados na área de influência do AHE Jirau Bacia do Rio Madeira
Beneficiário:Pedro de Magalhães Padilha
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 13/21297-1 - Metalotioneínas em peixes da região amazônica: estudo metalômico associado ao mercúrio
Beneficiário:Pedro de Magalhães Padilha
Linha de fomento: Auxílio à Pesquisa - Regular