Selective Targeting of Lymphoma Cells by Monoclonal Antibody Grafted onto Zwitterionic-Functionalized Nanoparticles

Autor(es)

Affonso de Oliveira, Jessica Fernanda ^{[1, 2]} ; da Silva, Raquel Frenedoso ^[2] ; Sousa Ribeiro, Iris Renata ^{[1, 2]} ; Saito, Angela ^[3] ; Melo-Hanchuk, Talita Diniz ^[4] ; Dias, Marilia Meira ^[3] ; Kobarg, Jorg ^{[4, 5]} ; Cardoso, Mateus Borba ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas UNICAMP, IQ, Caixa Postal 6154, BR-13083970 Campinas, SP - Brazil

^[2] CNPEM, Lab Nacl Nanotecnol LNNano, Caixa Postal 6192, BR-13083970 Campinas, SP - Brazil

^[3] Lab Nacl Biociencias LNBio, Caixa Postal 6192, BR-13083970 Campinas, SP - Brazil

^[4] Univ Estadual Campinas UNICAMP, IB, Programa Posgrad Biol Func & Mol, Dept Bioquim & Biol Tecidual, Caixa Postal 6154, BR-13083970 Campinas, SP - Brazil

^[5] Univ Estadual Campinas UNICAMP, Fac Ciencias Farmaceut, Caixa Postal 6154, BR-13083970 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PARTICLE & PARTICLE SYSTEMS CHARACTERIZATION; v. 37, n. 3 FEB 2020.
• Citações Web of Science: 1

Resumo

Nanomedicine is considered a promising alternative to improve cancer diagnosis and treatment. Particularly, the use of nanoparticles (NPs) has enabled the encapsulation of highly toxic anticancer drugs, facilitated ultimate targeting, and allowed tailoring of drug delivery. However, when in biological fluids, these NPs are coated by proteins which hide the targeting moieties and suppress the engineered biological outcome. Herein, how the Ki-1 monoclonal antibody (mAb) can preserve its targetability through grafting on the surface of zwitterionic-functionalized nanoparticles, is unveiled. Zwitterions, known for their stealth ability, are used to minimize unspecific NPs protein adsorption and consequently maintain mAb functionality. In this work, Ki-1 mAb is used as it recognizes TNFRSF8 (CD30(+)) transmembrane protein overexpressed on CD30(+) lymphoma cells such as L540 cells. While nonfunctionalized NPs show negligible toxic effects toward L540 cells, the Ki-1-functionalized structure demonstrates cytotoxicity, since they undergo cellular uptake, suggesting a receptor-mediated internalization. This dual-functionalization strategy provides a promising multifunctional nanoplatform toward future personalized medicine applications, minimizing unspecific protein adsorption on NPs and ensuring selective cancer cell targeting. (AU)

• Processo FAPESP: 14/22322-2 - Funcionalização de nanopartículas de sílica: aumentando a interação biológica
• Beneficiário: Mateus Borba Cardoso
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 15/25406-5 - Organizando a matéria: colóides formados por associação de surfactantes, polímeros e nanopartículas
• Beneficiário: Watson Loh
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 13/22429-9 - Síntese de nanopartículas de sílica mesoporosa funcionalizadas com anticorpos monoclonais: uma alternativa para o tratamento de linfomas Hodgkin (LH) e linfoma anaplásico de grandes células CD30+ (ALCL - CD30+)
• Beneficiário: Jessica Fernanda Affonso de Oliveira
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 17/21318-0 - Direcionamento de nanopartículas funcionalizadas em sistemas microfluídicos contendo múltiplos organoides
• Beneficiário: Iris Renata Sousa Ribeiro
• Modalidade de apoio: Bolsas no Brasil - Doutorado