Biological and microbiological behavior of calcium aluminate cement-based blend for filling of bone defects

Autor(es)

de Vasconcellos, Luana Marotta Reis ^[1] ; Campores, Kaike Lessa ^[1] ; de Alcantara Abdala, Julia Marinzeck ^[2] ; Vieira, Marilia Nanni ^[3] ; de Oliveira, Ivone Regina ^[2]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista UNESP, Inst Sci & Technol Sao Jose dos Campos, Dept Biosci & Oral Diag, Sao Jose Dos Campos, SP - Brazil

^[2] Univ Vale Paraiba, Inst Res & Dev, Sao Jose Dos Campos, SP - Brazil

^[3] Univ Estadual Paulista UNESP, Inst Sci & Technol Sao Jose dos Campos, Sao Jose Dos Campos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE; v. 31, n. 1 DEC 23 2019.
• Citações Web of Science: 2

Resumo

Calcium aluminate cement (CAC) as a biomaterial has been evaluated for its physical, mechanical and biocompatibility properties. Furthermore, the application of CAC for bone repair is due to its composition and coefficient of thermal expansion, which is similar to that of human bone. Thus, the aim of this study was to evaluate compositions of CAC-based blends as substitutes for bone defects. Five compositions of blends (alumina, zirconia, hydroxyapatite, tricalcium phosphate, chitosan), in addition to the base cement consisting of homogeneous CAC were evaluated as a substitute for bone repair. Additionally, the monotypic biofilm formation was assessed. Creation of a monocortical bone defect was performed on the femurs of rats, which were randomly filled with the different materials. The polymethylmethacrylate (PMMA) group was used as a control. All the animals were euthanized 04 weeks after the surgery procedure. Subsequently, computerized microtomography, histological and histomorphometric analyses were performed to verify the bone repair. To evaluate the formation of biofilms, reference strains of Staphylococcus aureus, Streptococcus mutans and Pseudomonas aeruginosa were cultured on the samples, and the biofilm formed was quantified by the MTT method. In the microtomography and histomorphometry results, it was observed that the blends exhibited better results than the control group, with statistically significant differences (p < 0.05) for alumina and zirconia blends. In the biofilm formation, a statistical difference (p < 0.05) in general was observed between the alumina blends and the control group (p < 0.05). It was concluded that CAC-based blends with alumina and zirconia are promising for use in fillings for bone repair. (AU)

• Processo FAPESP: 16/15032-3 - Caracterização in vitro e in vivo de blendas a base de cimento aluminoso visando aplicação no reparo e regeneração do tecido ósseo
• Beneficiário: Ivone Regina de Oliveira
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 17/22183-0 - Avaliação de blendas à base de cimento de aluminato de cálcio como substituto ósseo: estudo in vivo
• Beneficiário: Marília Nanni Vieira
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica