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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Anti-inflammatory and antioxidant activity improvement of lycopene from guava on nanoemulsifying system

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Autor(es):
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Adriany, Amorim [1] ; Jessica, Souza [1] ; Ana, Oliveira [2] ; Raimunda, Santos [1] ; Andreanne, Vasconcelos [3] ; Luan, Souza [4] ; Thiago, Araujo [4] ; Wanessa, Cabral [3] ; Maria, Silva [3] ; Ana, Mafud [5] ; Yvonne, Mascarenhas [5] ; Jand-Venes, Medeiros [4, 1] ; Jaco, Saraiva [6] ; Luis, Muehlmann [7] ; Selma, Kuckelhaus [3] ; Manuela, Pintado [2] ; Jose, R. Leite [3, 1]
Número total de Autores: 17
Afiliação do(s) autor(es):
[1] Univ Fed Piaui, Nucleo Pesquisa Biodiversidade & Biotecnol, BIOTEC, UFPI, CMRV, Parnaiba, PI - Brazil
[2] Univ Catolica Portuguesa, CBQF, Asprela Porto - Portugal
[3] Univ Brasilia, UnB, Fac Med, Area Morfol, Brasilia, DF - Brazil
[4] UFPI, LAFFEX, Lab Expt Physiopharmacol, Parnaiba, PI - Brazil
[5] Univ Sao Paulo, IFSC, Sao Carlos, SP - Brazil
[6] Medlig, Barretos, SP - Brazil
[7] Nanodynamics, Brasilia, DF - Brazil
Número total de Afiliações: 7
Tipo de documento: Artigo Científico
Fonte: JOURNAL OF DISPERSION SCIENCE AND TECHNOLOGY; v. 42, n. 5 FEB 2020.
Citações Web of Science: 2
Resumo

The nanoemulsion system can improve stability to antioxidant molecules when compared to the isolated molecule. Therefore, this study aimed to establish conditions on thermal stability, chemical characterization, antioxidant and anti-inflammatory activity of lycopene nanoemulsion. Different chemical assays were used to characterize the lycopene nanoemulsion (LN) and the purified lycopene from guava (LPG) as HPLC, UV-Vis, DLS, and ABTS to analyze the antioxidant activity of lycopene nanoemulsion. To test its thermal stability as an antioxidant in nanoemulsion it was used the thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC) techniques. The carrageenan-induced paw edema model in Swiss mice and histopathological analysis were used to evaluate anti-inflammatory activity. Emulsion with a mean droplet size of z-Average diameter 167.1 d.nm was produced. UV-vis detected lambda(max) = 447.1, 473.7, and 505.3 nm to lycopene from guava and HPLC-DAD analysis by C-30 at 472 nm identified the presence of lycopene in the nanoemulsion. This lycopene nanoemulsion presented thermal stability at room temperature, as well as on concentration when stored for one year. Also significantly inhibited (p < 0.05) the edema formation, with maximum inhibitory effect at 50 mg/kg by oral and intraperitoneal route and reduced neutrophil migration in paw tissue. The lycopene nanoemulsion can be a potential candidate for investigation and therapy of various diseases, including inflammation because this lycopene nanoemulsion presented therapeutics application for inflammatory diseases, and has also potential to be used as ointment and/or cream additive for topic applications. (AU)

Processo FAPESP: 14/02282-6 - Estudo da relação estrutura-atividade de alcalóides derivados da Pilocarpus microphyllus (Rutaceae) com ação contra Schistosoma mansoni
Beneficiário:Ana Carolina Mafud
Linha de fomento: Bolsas no Brasil - Pós-Doutorado