Role of embryonic origin on osteogenic potential and bone repair capacity of rat calvarial osteoblasts

Autor(es)

Souza, Alann Thaffarell Portilho ^[1] ; Lopes, Helena Bacha ^[1] ; Freitas, Gileade Pereira ^[1] ; Ferraz, Emanuela Prado ^[2] ; Oliveira, Fabiola Singaretti ^[1] ; Almeida, Adriana Luisa Goncalves ^[1] ; Weffort, Denise ^[1] ; Beloti, Marcio Mateus ^[1] ; Rosa, Adalberto Luiz ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Bone Res Lab, Sch Dent Ribeirao Preto, Av Cafe S-N, BR-14040904 Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Dept Maxillofacial Surg Prosthesis & Traumatol, Sch Dent, Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF BONE AND MINERAL METABOLISM; v. 38, n. 4 FEB 2020.
• Citações Web of Science: 0

Resumo

Introduction The aim of this study was to evaluate the in vitro osteogenic potential of osteoblasts from neural crest-derived frontal bone (OB-NC) and mesoderm-derived parietal bone (OB-MS) and the bone formation induced by them when injected into calvarial defects. Materials and methods Calvarial bones were collected from newborn Wistar rats (3-day old) and characterized as frontal and parietal prior to OB-NC and OB-MS harvesting. The cells were cultured, and several parameters of osteoblast differentiation were evaluated. These cells, or PBS without cells (control), were locally injected into 5-mm rat calvarial defects (5 x 10(6) cells/defect) and after 4 weeks bone formation was evaluated by morphometric and histological analyses. Results The characterization of frontal and parietal bones assured the different embryonic origin of both cell populations, OB-NC and OB-MS. The OB-NC presented higher proliferation while the OB-MS presented higher alkaline phosphatase (ALP) activity, extracellular matrix mineralization and gene expression of runt-related transcription factor 2, Alp, bone sialoprotein and osteocalcin revealing their high osteogenic potential. mu CT analysis indicated that there was higher amount of bone formation in defects injected with both OB-NC and OB-MS compared to the control. Moreover, the bone tissue formed by both cells displayed the same histological characteristics. Conclusions Despite the distinct in vitro osteogenic potential, OB-NC and OB-MS induced similar bone repair in a rat calvarial defect model. Thus, osteoblasts, irrespective of their in vitro osteogenic potential linked to embryonic origins, seem to be suitable for cell-based therapies aiming to repair bone defects. (AU)

• Processo FAPESP: 15/21439-6 - Células-tronco mesenquimais e osteoblastos: avaliação das assinaturas moleculares e do potencial para regenerar tecido ósseo
• Beneficiário: Adalberto Luiz Rosa
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 09/54142-5 - EMU: criação do Laboratório Multiusuários para análises de imagens tridimensionais de tecidos biológicos e biomateriais (LAB 3D BIO)
• Beneficiário: Adalberto Luiz Rosa
• Modalidade de apoio: Auxílio à Pesquisa - Programa Equipamentos Multiusuários