Contribution of Bone Marrow-derived Cells to Reparative Dentinogenesis Using Bone Marrow Transplantation Model

Autor(es)

Frozoni, Marcos ^[1] ; Marques, Marcelo Rocha ^[2] ; Hamasaki, Silvia Kaoru ^[1] ; Mohara, Nelson Tsutomu ^[1] ; Soares, Adriana de Jesus ^[3] ; Zaia, Alexandre Augusto ^[3]

Afiliação do(s) autor(es)

^[1] Inst Pesquisa Sao Leopoldo Mandic, Fac Sao Leopoldo Mandic, Area Endodontia, Campinas, SP - Brazil

^[2] Univ Estadual Campinas, Piracicaba Dent Sch, Dept Morphol, Div Histol & Embryol, Piracicaba, SP - Brazil

^[3] Univ Estadual Campinas, Piracicaba Dent Sch, Dept Restorat Dent, Div Endodont, Piracicaba, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF ENDODONTICS; v. 46, n. 3, p. 404-412, MAR 2020.
• Citações Web of Science: 1

Resumo

Introduction: The aim of this study was to analyze the contribution of bone marrow-derived cells (BMDCs) to reparative dentinogenesis using bone marrow transplantation (BMT) and pulp capping as an in vivo model. Methods: A chimeric mouse model was created through the injection of BMDCs expressing green fluorescent protein (GFP+ BMDCs) from C57BU6 GFP+ transgenic donor mice into irradiated C57BL/6 wild-type recipient mice (GFP- mice). These GFP-chimeric mice (containing transplanted GFP+ BMDCs) were subjected to microscopic pulp exposure and capping with white mineral trioxide aggregate (n = 18) or Biodentine (Septodont, St Maur-des-Fosses, France) (n = 18) in the maxillary first molar. Maxillary arches from GFP- chimeric mice (with the capped tooth) were isolated and histologically processed 5 (n = 9) and 7 (n = 9) weeks after BMT. Confocal laser microscopy and immunohistochemical analysis were performed to assess the presence of GFP+ BMDCs and the expression of dentin sialoprotein, an odontoblast marker, for those cells contributing to reparative dentinogenesis in the dental pulp. Results: Confocal laser microscopic analyses evidenced the presence of GFP+ BMDCs in close association with reparative dentin synthesized at the site of pulp exposure in GFP- mice 5 and 7 weeks after BMT. Immunohistochemical analysis revealed that GFP+ BMDCs in close association with reparative dentin expressed DSP, suggesting the contribution of nonresident GFP+ BMDCs to reparative dentinogenesis. Conclusions: These data suggest the presence of nonresident BMDCs in reparative dentinogenesis and its contribution to dental pulp regeneration in the pulp healing process. (AU)

• Processo FAPESP: 14/18623-7 - Estudo das células e tecidos envolvidos no processo de reparação e de revitalização / revascularização da polpa dental
• Beneficiário: Alexandre Augusto Zaia
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/50251-0 - Estudo das células envolvidas no processo de reparação da polpa dental: análise laboratorial
• Beneficiário: Marcos Roberto dos Santos Frozoni
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado