Systemic and local effects of radiotherapy: an experimental study on implants placed in rats

Autor(es):Mostrar menos -

Vegian, Mariana Raquel da Cruz ^[1] ; Costa, Bruno Cesar Almeida ^[1] ; Santana-Melo, Gabriela de Fatima ^[1] ; Godoi, Fernanda Herrera Costa ^[1] ; Kaminagakura, Estela ^[1] ; Tango, Rubens Nisie ^[2] ; do Prado, Renata Falchete ^[1] ; de Oliveira, Luciane Dias ^[1] ; Federico, Claudio Antonio ^[3] ; Avelino, Sarah de Oliveira Marco ^[4] ; Neves, Rafael Marques ^[1] ; de Vasconcellos, Luana Marotta Reis ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ Unesp, Inst Sci & Technol Sao Jose dos Campos, Dept Biosci & Oral Diag, Ave Engn Francisco Jose Longo 777, BR-12245000 Sao Jose Dos Campos, SP - Brazil

^[2] Sao Paulo State Univ Unesp, Inst Sci & Technol Sao Jose dos Campos, Dept Dent Mat & Prosthodont, Sao Jose Dos Campos, SP - Brazil

^[3] Technol Inst Aeronaut, Inst Adv Studies, Dept Aerosp Sci & Technol, Sao Jose Dos Campos, SP - Brazil

^[4] Sao Paulo State Univ Unesp, Inst Sci & Technol Sao Jose dos Campos, Sao Jose Dos Campos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: CLINICAL ORAL INVESTIGATIONS; v. 24, n. 2, p. 785-797, FEB 2020.
• Citações Web of Science: 0

Resumo

Objectives Evaluate the modulating effect of ionizing radiation, blood cytokine levels, and bone remodeling of the interface around the implant to understand the radiation mechanisms which can impair the implants receptor site. Material and methods Sixty rats were submitted to grade V titanium implants in the femurs and were divided into the following groups: no-irradiation (N-Ir): control group with implant only; early-irradiation (E-Ir): implant + irradiation after 24 h; late-irradiation (L-Ir): implant + irradiation after 4 weeks; and previous-irradiation (P-Ir): irradiation + implant after 4 weeks. The animals in the E-Ir, L-Ir, and P-Ir groups were irradiated in two fractional stages of 15 Gy. At 3 days, 2 weeks, and 7 weeks after the final procedure, five animals were randomly euthanized per group. Serum levels of TNF-\& x251;, IL-1 beta, TGF-beta, IL-6, M-CSF, and IL-10 were measured from blood collected prior to euthanasia using the ELISA test. The pieces containing the implants were subjected to immunohistochemical labeling using the tartrate acid resistant to phosphatase, osteocalcin, and caspase-3 markers and mCT. The ANOVA test was used for statistical analysis, and the Tukey multiple comparison test (p < 0.05) was applied. Results The results indicated that ionizing radiation modifies the production of pro- and anti-inflammatory serum cytokines, the expression of proteins involved in bone remodeling and cellular apoptosis, as well as changes in bone formation. Conclusions The results suggests that a longer period between radiotherapy and implant placement surgery when irradiation occurs prior to implant installation would allow the recovery and renewal of bone cells and avoid future failures in osseointegration. (AU)

• Processo FAPESP: 17/04389-0 - Efeito modulador da radiação ionizante sobre citocinas sanguíneas em ratos submetidos a cirurgia de implantes
• Beneficiário: Sarah de Oliveira Marco Avelino
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 17/00543-5 - Efeitos da radioterapia na interface osso-implante e nos tecidos moles circunjacentes
• Beneficiário: Rafael Marques Neves
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 16/19707-5 - Efeito modulador da radiação ionizante sobre citocinas sanguíneas e células do tecido ósseo presentes na interface osso-implante
• Beneficiário: Mariana Raquel da Cruz Vegian
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 15/24986-8 - Efeitos da radioterapia na neoformação óssea, osseointegração, tecidos circunjacentes e células mesenquimais
• Beneficiário: Luana Marotta Reis de Vasconcellos
• Modalidade de apoio: Auxílio à Pesquisa - Regular