S-nitrosothiol-terminated poly(vinyl alcohol): Nitric oxide release and skin blood flow response

Autor(es)

Giglio, Leonardo P. ^[1] ; Picheth, Guilherme F. ^[1] ; Lovschall, Kaja Borup ^[2] ; Zelikin, Alexander N. ^{[2, 3]} ; de Oliveira, Marcelo G. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Unicamp, Inst Chem, BR-13083861 Campinas, SP - Brazil

^[2] Aarhus Univ, Dept Chem, DK-8000 Aarhus C - Denmark

^[3] Aarhus Univ, INano Interdisciplinary Nomsci Ctr, DK-8000 Aarhus C - Denmark

• Tipo de documento: Artigo Científico
• Fonte: NITRIC OXIDE-BIOLOGY AND CHEMISTRY; v. 98, p. 41-49, MAY 1 2020.
• Citações Web of Science: 0

Resumo

Polymeric biomaterials capable of delivering nitric oxide (NO) topically can be used to enhance skin blood flow (SkBF) and accelerate wound healing. Herein, we used reversible addition-fragmentation chain transfer radical (RAFT) polymerization to synthesize the first poly(vinyl alcohol) (PVA) functionalized with terminal NO-releasing S-nitrosothiol (RSNO) groups for topical NO delivery. This strategy was based on the synthesis of a precursor amino-terminated PVA (PVA-NH2), which was next functionalized with iminothiolane yielding 4-imino-4-amino-PVA-butane-1-thiol (PVA-SH), and finally S-nitrosated yielding S-nitroso 4-imino-4-amino-PVAbutane-1-thiol (PVA-SNO). Real-time chemiluminescence NO detection showed that blended films of pure PVA with PVA-SNO with mass ratios 30:70, 50:50 and 70:30 release NO with initial rates ranging from 1 to 12 nmol g(-1) min(-1), and lead to a 2 to 10-fold dose-response increase in the SkBF, after topical application on the ventral forearm of volunteers. These results show that PVA-SNO is a potential platform for topical NO delivery in biomedical applications. (AU)

• Processo FAPESP: 17/04622-7 - Fibras eletrofiadas de PVA-GSNO: preparação e atividade biológica
• Beneficiário: Leonardo Pinheiro Giglio
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Iniciação Científica
• Processo FAPESP: 16/02414-5 - Biomateriais absorvíveis e tópicos para a liberação localizada de óxido nítrico
• Beneficiário: Marcelo Ganzarolli de Oliveira
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 17/19253-7 - Hidrogéis termosensíveis de Pluronic F127 funcionalizados com grupos terminais liberadores de óxido nítrico
• Beneficiário: Guilherme Fadel Picheth
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado