Bidens pilosa (Black Jack) Standardized Extract Ameliorates Acute TNBS-induced Intestinal Inflammation in Rats

Autor(es)

Quaglio, V, Ana E. ; Cruz, Vinicius M. ^[1] ; Almeida-Junior, Luiz D. ^[1] ; Costa, Celso A. R. A. ^[1] ; Di Stasi, Luiz C. ^[1]

Afiliação do(s) autor(es)

^[1] Quaglio, Ana E., V, Sao Paulo State Univ, Lab Phytomed Pharmacol & Biotechnol PhytoPharmaTe, Dept Pharmacol, UNESP, Inst Biosci, Rua Prof Dr Antonio Celso Wagner Zanin 250, BR-18618689 Botucatu, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Planta Medica; v. 86, n. 5, p. 319-330, MAR 2020.
• Citações Web of Science: 0

Resumo

Bidens pilosa is an herb popularly used to treat inflammation, hemorrhoids, fever, and gastric ulcers with reported pharmacological activities and chemical composition that sustain its selection as a potential intestinal anti-inflammatory product. Based on this, we examined the effects of a B. pilosa fatty acid-standardized supercritical preparation on the intestinal inflammatory process induced by trinitrobenzenesulphonic acid in rats, using either preventative or curative treatments. We also investigated the safety of plant extract by acute and sub-chronic toxicological analysis. The intestinal anti-inflammatory activity was related to modulation of the immune response, increasing IL-10 production and reducing IL-1 beta , IL-6, and TNF- alpha level, the oxidative stress, and the MUC production in the inflamed colon. Optic, scanning, and transmission electron microscopy (TEM) analysis supported the beneficial effects promoted by B. pilosa , which were closely related to downregulation of heparanase, Hsp70, Mapk 3, and NF- kappa B signaling and with the presence of long-chain fatty acids in extract. Our data suggest that B. pilosa supercritical preparation is a chemically standardized preparation potentially useful as complementary intestinal anti-inflammatory agent to treat inflammatory bowel disease. (AU)

• Processo FAPESP: 11/50824-4 - Avaliacao dos mecanismos de acao antiinflamatoria intestinal de esculetina, 4-metilesculetina e esculina sobre novos marcadores moleculares.
• Beneficiário: Aline Witaicenis Fantinati
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 17/03959-8 - MicroRNAs como marcadores de diagnóstico e prognóstico da Retocolite Ulcerativa e Doença de Crohn em pacientes portadores de doença inflamatória intestinal
• Beneficiário: Ana Elisa Valencise Quaglio
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 15/15267-8 - Doença inflamatória intestinal (DII): novas abordagens de diagnóstico e modulação da microbiota intestinal em pacientes com retocolite ulcerativa
• Beneficiário: Luiz Claudio Di Stasi
• Modalidade de apoio: Auxílio à Pesquisa - Temático