Genomic evidence of yellow fever virus in Aedes scapularis, southeastern Brazil, 2016

Autor(es):Mostrar menos -

Cunha, Mariana Sequetin ^[1] ; Faria, Nuno Rodrigues ^[2] ; Caleiro, Giovana Santos ^{[1, 3]} ; Candido, Darlan Silva ^[2] ; Hill, Sarah C. ^[2] ; Claro, Ingra Morales ^[3] ; da Costa, Antonio Charlys ^[3] ; Nogueira, Juliana Silva ^[1] ; Maeda, Adriana Yurika ^[1] ; da Silva, Fernanda Gisele ^[1] ; de Souza, Renato Pereira ^[1] ; Spinola, Roberta ^[4] ; Tubaki, Rosa Maria ^[5] ; Tironi de Menezes, Regiane Maria ^[5] ; Abade, Leandro ^[2] ; Mucci, Luis Filipe ^[6] ; Sampaio Tavares Timenetsky, Maria do Carmo ^[1] ; Sabino, Esther ^[3]

Afiliação do(s) autor(es)

^[1] Adolfo Lutz Inst, Sao Paulo, SP - Brazil

^[2] Univ Oxford, Dept Zool, Oxford - England

^[3] Univ Sao Paulo, Inst Trop Med, Sao Paulo, SP - Brazil

^[4] Ctr Vigilancia Epidemiol Prof Alexandre Vranjac, Sao Paulo, SP - Brazil

^[5] Superintendence Control Endem Dis, Sao Paulo, SP - Brazil

^[6] Superintendence Control Endem Dis, Taubate, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Acta Tropica; v. 205, MAY 2020.
• Citações Web of Science: 0

Resumo

The southeastern region of Brazil has recently experienced the largest yellow fever disease outbreak in decades. Since July 2016 epizootic events were reported in Sao Paulo state's north region, where 787 Culicidae were captured as part of public health surveillance efforts and tested using real-time quantitative PCR. One Aedes scapularis pool collected in November 2016 in an agriculture area in Urupes city tested positive for YFV-RNA. Using a validated multiplex PCR approach we were able to recover a complete virus genome sequence from this pool. Phylogenetic analysis of the novel strain and publicly available data indicates that the belongs to the South American genotype 1 clade circulating in Sao Paulo state and is basal to the recent outbreak clade in southeast Brazil. Our findings highlight the need of additional studies, including vector competence studies, to disentangle the role of Aedes scapularis in yellow fever transmission in the Americas. (AU)

• Processo FAPESP: 17/00021-9 - Metagenômica viral da Dengue, Chikungunya e Zika: acompanhar, explicar e prever a transmissão e distribuição espaço-temporal no Brasil
• Beneficiário: Antonio Charlys da Costa
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 18/14389-0 - Centro Conjunto Brasil-Reino Unido para Descoberta, Diagnóstico, Genômica e Epidemiologia de Arbovírus (CADDE)
• Beneficiário: Ester Cerdeira Sabino
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 16/01735-2 - Metagenômica viral de Dengue, Chikungunya e Zika vírus: acompanhar, explicar e prever a transmissão e distribuição espaço-temporal no Brasil
• Beneficiário: Ester Cerdeira Sabino
• Modalidade de apoio: Auxílio à Pesquisa - Regular